Recombinant Protein Expression in Rhodococcus species
Abstract Producing recombinant bacterial proteins is essential for elucidating their structure and function, making efficient overexpression systems a key requirement. Escherichia coli is widely used for this purpose, particularly for bacterial proteins, but certain targets remain challenging to express or obtain as soluble protein.
Michael Akintubosun, Melanie A. Higgins
wiley +1 more source
Soybean‐derived exosome‐like nanovesicles suppress lipid accumulation of adipocytes via UCP1/PGC‐1α mediated thermogenesis pathway and boosting mitochondrial function. ABSTRACT Obesity has become an important public health concern in modern society. Plant‐derived exosome‐like nanovesicles are emerging as candidates for obesity intervention.
Zhenzhu Zhu +5 more
wiley +1 more source
Molecular characterization of methicillin-resistant Staphylococcus aureus strains by spa typing and pulsed field gel electrophoresis methods. [PDF]
Güven Gökmen T +3 more
europepmc +1 more source
Steviol Glycosides (E960) from Stevia rebaudiana: Technological Innovations, Safety Assessment, Regulatory Aspects and their Role in Sustainable Food Systems. ABSTRACT Stevia (Stevia rebaudiana Bertoni) is a small perennial herb widely recognized as a natural sweetener.
Aswin Sakthivel Manoharan +1 more
wiley +1 more source
Advancements in Mass Spectrometry‐Based Glycomics in Food and Nutritional Science
ABSTRACT This review highlights advancements in mass spectrometry (MS)‐based glycomics in food and nutritional science. Carbohydrates, which are vital for human health, exhibit complex structures, making their analysis challenging. MS has become an indispensable tool for elucidating the structures of carbohydrates, including glycans, through soft ...
JaeHui Song +3 more
wiley +1 more source
Genotyping of commensal Neisseria spp strains by pulsed-field gel electrophoresis and 16S rRNA gene sequencing. [PDF]
Mechergui A, Achour W, Ben Hassen A.
europepmc +1 more source
Mass spectrometry proteomics for studying mitostasis
Abstract Maintaining mitochondrial integrity and function is fundamental to cellular homeostasis. Cells rely on coordinated protein quality control (QC) systems—including intricate chaperone‐protease networks, the ubiquitin‐proteasome system, and cytosolic surveillance pathways—that together form a dynamic, cell‐wide mitostasis network governing the ...
Lakshita Sharma +5 more
wiley +1 more source
Analysis of Salmonella enterica Serovar Typhi by Outer Membrane Protein (OMP) Profiling, Random Amplification of Polymorphic DNA (RAPD) and Pulsed Field Gel Electrophoresis (PFGE). [PDF]
Kumar Y, Mani KR, Tahlan AK.
europepmc +1 more source
Evidence for a Two‐Step Model for Activation of GPR25 by the Chemoattractant CXCL17
ABSTRACT CXCL17 was recently reported to activate GPR25, a receptor expressed by T‐regulatory cells. Although classified as a chemokine, the activity of CXCL17 is ablated by minor C‐terminal truncation, suggesting a novel mode of receptor activation. We set out to test this hypothesis by mutagenesis. GPR25 was expressed in the murine pre‐B cell line L1.
Wuqing Yang +2 more
wiley +1 more source
Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India. [PDF]
Yadav JP +6 more
europepmc +1 more source

