Results 171 to 180 of about 1,815,431 (234)

Ultrasound-Assisted Extraction of Polysaccharides from Mulberry Leaves Using Response Surface Methodology: Purification and Component Identification of Extract. [PDF]

Molecules
Wang T, Zou X, Zhang H, Li J, Peng X, Ju R, Jia Z, Wen Z, Li C.   +8 more
europepmc   +1 more source

KCS1 and VIP1, the genes encoding yeast phosphoinositol pyrophosphate synthases, are required for Ca2+‐mediated response to dimethylsulfoxide (DMSO)

FEBS Open Bio, EarlyView.
Ca2+‐mediated response to DMSO was investigated in Saccharomyces cerevisiae cells expressing Ca2+‐dependent aequorin. Cell exposure to DMSO induced a cytosolic Ca2+ wave dependent on the integrity of the Cch1/Mid1 channel. Deletion of KCS1 or VIP1 genes encoding the phosphoinositol pyrophosphate (PP‐IP) synthases suppressed the DMSO‐induced Ca2 ...
Larisa Ioana Gogianu, Lavinia Liliana Ruta, Claudia Valentina Popa, Simona Ghenea, Ileana Cornelia Farcasanu   +4 more
wiley   +1 more source

AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing. [PDF]

Commun Biol
Saraithong P, Krajcarski P, Kusaka Y, Yamada M, Matsumoto J, Cunningham H, Salih S, Jones D, Baddhan D, Hausner C, Anumonwo J, Rosenzweig A, Navarro MM, Diaz LV, Criscione J, Kim DH, Herron TJ.   +16 more
europepmc   +1 more source

Protein O‐glycosylation in the Bacteroidota phylum

FEBS Open Bio, EarlyView.
Species of the Bacteroidota phylum exhibit a unique O‐glycosylation system. It modifies noncytoplasmic proteins on a specific amino acid motif with a shared glycan core but a species‐specific outer glycan. A locus of multiple glycosyltransferases responsible for the synthesis of the outer glycan has been identified.
Lonneke Hoffmanns, Dennis Svedberg, André Mateus   +2 more
wiley   +1 more source

Influence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles. [PDF]

Front Mol Biosci
Gookin TE, Chakravorty D, Assmann SM.
europepmc   +1 more source

Neurotoxic amyloid β‐peptide and tau produce cytokine‐like effects on PMCA in glioblastoma cell lines, enhancing its activity and isoforms expression

FEBS Open Bio, EarlyView.
Two biomarkers of Alzheimer's disease, amyloid β‐peptide (Aβ) and tau, induce the transformation of U‐251 and other glioblastoma cell lines into neurotoxic A1‐like reactive astrocytes. This transformation is produced by cytokines and is followed by upregulation of PMCA activity and isoform expression, and is closely associated with inflammation, as ...
María Berrocal, Alberto Alvarez‐Barrientos, Ana M. Mata   +2 more
wiley   +1 more source

Protocol for producing brain-derived neurotrophic factor and neurotrophin-4 in their pro and active form in Escherichia coli. [PDF]

STAR Protoc
Holzner C, Böttinger K, Huber CG, Dall E, Brandstetter H.   +4 more
europepmc   +1 more source

Iron‐dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP‐1 monocytes

FEBS Open Bio, EarlyView.
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník   +4 more
wiley   +1 more source

An approach for coherent periodogram averaging of tilt‐series data for improved contrast transfer function estimation

FEBS Open Bio, EarlyView.
The contrast transfer function (CTF) is an imaging aberration that is a major resolution‐limiting factor in cryo‐electron microscopy (cryo‐EM). Precise CTF estimation is key to overcoming this limitation, but is particularly challenging in cryo‐electron tomography (cryo‐ET) data. Here, we present an approach for using geometric information to assist in
Sagar Khavnekar, William Wan
wiley   +1 more source

Chemical linkers switch triglycerol detergents from bacterial protein purification to mild antibiotic amplification. [PDF]

Commun Chem
Singh AK, Seewald M, Schade B, Zoister C, Haag R, Urner LH.   +5 more
europepmc   +1 more source