Results 241 to 250 of about 2,151,988 (346)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Protocol to purify fatty acid metabolites with two approaches for quantification using liquid chromatography-mass spectrometry. [PDF]

STAR Protoc
Hashidate-Yoshida T, Yamada M, Nagata K, Kihara Y, Kita Y, Shindou H.   +5 more
europepmc   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Biochemical Engineering Perspective on cGAS: From Enzyme Discovery to Potential Industrial Application. [PDF]

Chembiochem
Fataeri M, Rosenthal K.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Comparative assessment of image quality and radiation dose: spectral purification computed tomography (Sn100kV) with adaptive iterative reconstruction for tuberculosis follow-up. [PDF]

J Thorac Dis
Yuan C, Yang J, Huang Y, Qin C, Wang C, Feng H, Zhong J, He C, Dan H.   +8 more
europepmc   +1 more source

Enantio-purification et mécanochimie : une combinaison innovante et efficace

Chrystal Lopes, Clément Brandel, Oleksii Shemchuk, Tom Leyssens, Yohann Cartigny, Valérie Dupray   +5 more
openalex   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Purification of murine cortex mitochondria using a modified magnetic bead isolation method. [PDF]

MethodsX
Josic E, Ujevic A, Puljko B, Maček Hrvat N, Lipovcic A, Kalanj-Bognar S, Mlinac-Jerkovic K.   +6 more
europepmc   +1 more source

An efficient methodology for the purification of date palm peroxidase: Stability comparison with horseradish peroxidase (HRP)

, 2018
Moneera Saud Al-Bagmi, Mohd Shahnawaz Khan, Mohamad Alhasan Ismael, Abdulrahman M. Alsenaidy, Abir Ben Bacha, Fohad Mabood Husain, Salman Alamery   +6 more
openalex   +1 more source