Advanced NanoBiomed Research, EarlyView.We developed an assay to distinguish cellular binding from internalization. Compatible with microscopy and high‐throughput screening, the method identifies ligand‐mediated uptake. Applying top candidates to a protein‐based DNA carrier enhanced transfection efficiency, providing a rational strategy to improve non‐viral gene delivery systems.
David Scherer +5 more
wiley +1 more source
A Cholesterol Analogue for Cell‐Surface Enzyme Display
Angewandte Chemie, EarlyView.We report a non‐genetic strategy for bacterial cell surface enzyme immobilisation using cholesterol‐based artificial lipids containing Ni2+‐NTA binding groups that can integrate into biological membranes. The engineered cells can capture His‐tagged enzymes directly from cell lysates while preserving intracellular and surface activity, allowing for ...
Vasco F. Batista +3 more
wiley +2 more sources
Erratum for Fecteau et al., "Primary production by the purple nonsulfur bacterium <i>Rhodopila globiformis</i> in an acidic, moderately sulfidic warm spring". [PDF]
Appl Environ MicrobiolFecteau KM +8 more
europepmc +1 more source
Safety and Feasibility of Magnetic Separation‐Based Blood Purification
Advanced NanoBiomed Research, EarlyView.We present the preclinical biological safety and translational feasibility of a magnetic nanoparticle‐based extracorporeal blood purification platform for removal of disease‐causing blood factors. ISO 10993‐compliant studies and exaggerated worst‐case exposure models demonstrate efficient nanoparticle extraction (≥99.9%), favorable biocompatibility ...
Carlos A. Mora +11 more
wiley +1 more source
Structural and Functional Siderophore Remodeling by Enzymatic Delipidation
Angewandte Chemie, EarlyView.A surprising functional switch in bacterial siderophores was discovered through genome mining and the analysis of environmental and pathogenic Pandoraea species. Pandorachelin B, a lipocyclopeptide that promotes swarming motility, is cleaved by a specialized acylase, yielding a ring‐contracted, homodetic cyclopeptide, pandorachelin A, which exhibits ...
Elena Herzog +5 more
wiley +2 more sources
Probiotics use environmentally friendly calcium lignosulfonate as an energy source to MICP-acting on concrete and soil remediation. [PDF]
Front MicrobiolYan ZJ +10 more
europepmc +1 more source
Angewandte Chemie, EarlyView.Here, we enhance the electrochemiluminescence imaging of cellular spheroids using [Ru(bpy)3]2+/TPrA chemistry. Our strategy, which involves pre‐incubation with TPrA, enables high‐resolution, long‐term visualization of cellular spheroids without phototoxicity.
Vanshika Gupta +5 more
wiley +2 more sources
Bioactive pigment production by marine bacteria from Colombian estuarine and deep-sea environments. [PDF]
Sci RepRíos-López LG +4 more
europepmc +1 more source
Angewandte Chemie, EarlyView.Coupling biosynthetic noncanonical amino acid production with genetic code expansion enables site‐specific incorporation of azatryptophans into PET‐degrading enzymes (PETases). By isosteric single‐atom editing of a conserved tryptophan, AzaPETases break the activity–stability trade‐off, delivering higher catalytic efficiency at elevated temperature and
Elwy H. Abdelkader +3 more
wiley +2 more sources
Genomic insights into multidrug-resistant bacteria: Emergence of <i>mcr-1.1</i> in <i>Escherichia coli</i>, <i>bla</i><sub>KPC-2</sub> in <i>Klebsiella pneumoniae</i> and <i>Serratia marcescens</i> from clinical settings. [PDF]
VirulenceHameed MF +4 more
europepmc +1 more source