Results 261 to 270 of about 48,907 (300)

Binding of a photoaffinity analogue of pyridoxal to pyridoxal kinase [PDF]

European Journal of Biochemistry, 1990
The binding of pyridoxal analogues to the structural domains of pyridoxal kinase was studied by fluorescence spectroscopy and chromatographic techniques. Two fragments of 24 and 16 kDa, arising from limited proteolysis of the native enzyme, were separated by ion‐exchange chromatography and used for binding studies with pyridoxal oxime.
Glen M. Scholz, Francis Kwok, Jorge E. Churchich   +2 more
openaire   +2 more sources

Some of the next articles are maybe not open access.

Related searches:

The interactions between biogenic amines and pyridoxal, pyridoxal phosphate, and pyridoxal kinase

Archives of Biochemistry and Biophysics, 1972
Abstract The effect of biogenic amines on pyridoxal kinase activity has been investigated in vitro . Experiments with a purified beef brain pyridoxal kinase indicate that 3,4-dihydroxyphenylalanine (dopa), dopamine, norepinephrine, and serotonin inhibit the reaction, while tyrosine, tryptophan, and 5-hydroxytryptophan (5-HTP) have no effect. However,
R.L. Meneely, Warren F. Diven, M.R. Grever, J.T. Neary   +3 more
openaire   +3 more sources

On the mechanism of Escherichia coli pyridoxal kinase inhibition by pyridoxal and pyridoxal 5′-phosphate

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2015
Pyridoxal 5'-phosphate (PLP), the catalytically active form of vitamin B6, plays a crucial role in several cellular processes. In most organisms, PLP is recycled from nutrients and degraded B6-enzymes in a salvage pathway that involves pyridoxal kinase (PLK), pyridoxine phosphate oxidase and phosphatase activities.
DI SALVO, Martino Luigi, Nogués I, PARRONI, ALESSIA, Tramonti A, MILANO, TERESA, PASCARELLA, Stefano, CONTESTABILE, Roberto   +6 more
openaire   +3 more sources

Fluorometric assay of pyridoxal and pyridoxal 5′-phosphate

Analytical Biochemistry, 1979
Abstract A new and very sensitive fluorometric method for the determination of pyridoxal and pyridoxal 5′-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its 5′-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4,5 to 5,0.
K. Dakshinamurti, M.S. Chauhan
openaire   +3 more sources

Inhibition of gaba binding by pyridoxal and pyridoxal phosphate

International Journal of Biochemistry, 1980
Abstract 1. 1. GABA exerts its neuroinhibitory effects by binding to a specific receptor on the post-synaptic membrane. 2. 2. Since both GABA and vitamin B 6 have been implicated in convulsion, the effects of B 6 vitamers on Na-independent binding of GABA was investigated by incubating synaptic membrane suspensions for 5 min at 4°C in 1 ml ...
Borpit Klangkalya, Jean D. Deupree, Manuchair Ebadi   +2 more
openaire   +3 more sources

Measurement of pyridoxal 5′-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children

Clinica Chimica Acta, 2017
We quantified pyridoxal 5'-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers.CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using
Tomoyuki Akiyama, Mari Akiyama, Yumiko Hayashi, Takashi Shibata, Yoshiyuki Hanaoka, Soichiro Toda, Katsumi Imai, Shin-ichiro Hamano, Tohru Okanishi, Harumi Yoshinaga, Katsuhiro Kobayashi   +10 more
openaire   +3 more sources

New Pathway of Conversion of Pyridoxal to 4-Pyridoxic Acid

Enzyme, 1976
The only enzyme demonstrated so far to catalyze the formation of 4-pyridoxic acid, the final excretory product of vitamin B6, was aldehyde oxidase. In this paper we have presented an evidence that another enzyme, NAD+-dependent aldehyde dehydrogenase, is capable of catalyzing this reaction.
M Stanulović, V Jeremić, S Chaykin, V Leskovac   +3 more
openaire   +3 more sources

The triplet state of pyridoxal

Biochemical and Biophysical Research Communications, 1968
Abstract The absorption spectra, as well as the relative distribution of the structures of pyridoxal in aqueous solution, have been investigated in several laboratories ( Williams and Neilands, 1954 ; Metzler and Snell, 1955 ; Anderson and Martell, 1964) .
Jorge E. Churchich, John E. Wampler
openaire   +3 more sources