Functional Characterization of Four Putative δ1-Pyrroline-5-Carboxylate Reductases from Bacillus subtilis [PDF]
Frontiers in Microbiology, 2017 In most living organisms, the amino acid proline is synthesized starting from both glutamate and ornithine. In prokaryotes, in the absence of an ornithine cyclodeaminase that has been identified to date only in a small number of soil and plant bacteria ...
Giuseppe Forlani +4 more
doaj +3 more sources
Emerging Roles of De Novo Proline Biosynthesis in Human Diseases. [PDF]
FASEB BioadvDe novo proline synthesis, a multi‐step biochemical pathway starting from glutamate, supplies essential protein building blocks. It supports redox balance, cell proliferation, signal transduction, and nucleotide and protein biosynthesis. This tightly regulated pathway, governed by conserved mechanisms, is disrupted in diseases such as cancer and ...
Pei E, Ma J.
europepmc +2 more sources
Pyrroline-5-carboxylate reductase in human erythrocytes. [PDF]
Journal of Clinical Investigation, 1981 Pyrroline-5-carboxylate reductase, which converts pyrroline-5-carboxylate to proline, has been identified in human erythrocytes. The level of pyrroline-5-carboxylate reductase activity in these cells is comparable to the activity levels of major erythrocyte enzymes.
G C, Yeh, S C, Harris, J M, Phang
openaire +2 more sources
Osmoregulation of a Pyrroline-5-Carboxylate Reductase Gene in Arabidopsis thaliana [PDF]
Plant Physiology, 1993 In Arabidopsis thaliana (L.) Heynh. proline can account for up to 20% of the free amino acid pool after salt stress. Proline accumulation occurs in plants mainly by de novo synthesis from glutamate. The last step of the proline biosynthetic pathway is catalyzed by pyrroline-5-carboxylate (P5C) reductase.
M. Van Montagu +2 more
core +4 more sources
Purified Human Erythrocyte Pyrroline-5-carboxylate Reductase
Journal of Biological Chemistry, 1989 Pyrroline-5-carboxylate reductase catalyzes the final step in proline synthesis by NAD(P)H-dependent reduction of pyrroline-5-carboxylate. We have purified and characterized this enzyme from human erythrocytes. Purification to homogeneity (approximately 600,000-fold) was accomplished by sonication, ultracentrifugation, 2',5'-ADP-Sepharose affinity ...
M J Merrill, G C Yeh, J M Phang
openaire +2 more sources
Associated bacteria affect sexual reproduction by altering gene expression and metabolic processes in a biofilm inhabiting diatom [PDF]
, 2019 Diatoms are unicellular algae with a fundamental role in global biogeochemical cycles as major primary producers at the base of aquatic food webs. In recent years, chemical communication between diatoms and associated bacteria has emerged as a key factor
Bilcke, Gust +9 more
core +2 more sources
Purification to Homogeneity of Pyrroline-5-Carboxylate Reductase of Barley [PDF]
Plant Physiology, 1986 An enzyme has been purified to homogeneity from barley seedlings which has ;proline dehydrogenase' and the pyrroline-5-carboxylic acid reductase activities. The purification achieved is 39,000-fold as calculated from the proline dehydrogenase activity. The subunit molecular weight of the protein is 30 kilodaltons.
R, Krueger +3 more
openaire +2 more sources
N-Alkyl-α-amino acids in Nature and their biocatalytic preparation [PDF]
, 2019 PWS would like to acknowledge the European Union for his current funding: “This project has received funding from the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement No 665919”.N-alkylated-α ...
Hyslop, Julia F. +4 more
core +2 more sources
Reaction of O2 with a di-iron protein generates a mixed valent Fe2+/Fe3+ center and peroxide [PDF]
, 2019 The gene encoding the cyanobacterial ferritin SynFtn is up-regulated in response to copper stress. Here, we show that, while SynFtn does not interact directly with copper, it is highly unusual in several ways.
Bradley, Justin M. +9 more
core +2 more sources
Comprehensive transcriptome analysis unravels the existence of crucial genes regulating primary metabolism during adventitious root formation in Petunia hybrida [PDF]
, 2014 To identify specific genes determining the initiation and formation of adventitious roots (AR), a microarray-based transcriptome analysis in the stem base of the cuttings of Petunia hybrida (line W115) was conducted.
Ahkami, Amirhossein +10 more
core +4 more sources