ChemistryEurope, Volume 3, Issue 5, September 18, 2025.A standalone enzyme, derived from the reductase domain of a polyketide synthase‐nonribosomal peptide synthetase hybrid enzyme, is identified as a pyrroline carboxylate reductase. This enzyme is involved in the biosynthesis of 3‐methylproline, an amino acid building block of a unique tricyclic pyrrolizidinone structure of a fungal metabolite ...
Naoki Kato +6 more
wiley +1 more source
One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst [PDF]
, 2018 Mindt M, Risse JM, Gruß H, Sewald N, Eikmanns BJ, Wendisch VF. One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst. Scientific Reports.
Eikmanns, Bernhard J. +5 more
core +3 more sources
Cloning human pyrroline-5-carboxylate reductase cDNA by complementation in Saccharomyces cerevisiae.
Journal of Biological Chemistry, 1992 Pyrroline-5-carboxylate reductase (EC 1.5.1.2) catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. We cloned a human pyrroline-5-carboxylate reductase cDNA by complementation of proline auxotrophy in a Saccharomyces cerevisiae mutant strain, DT1100. Using a HepG2 cDNA library in a yeast expression vector, we screened 10(5)
K M, Dougherty, M C, Brandriss, D, Valle
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Tropane and Granatane Alkaloid Biosynthesis: A Systematic Analysis [PDF]
, 2016 The tropane and granatane alkaloids belong to the larger pyrroline and piperidine classes of plant alkaloids, respectively. Their core structures share common moieties and their scattered distribution among angiosperms suggest that their biosynthesis may
Chavez, Benjamin +4 more
core +3 more sources
Evidence for Hysteretic Substrate Channeling in the Proline Dehydrogenaseand ∆\u3csup\u3e1\u3c/sup\u3e-Pyrroline-5-carboxylate Dehydrogenase Coupled Reaction of Proline UtilizationA(PutA) [PDF]
, 2014 Background: PutA from Escherichia coli is a bifunctional enzyme and transcriptional repressor in proline catabolism. Results: Steady-state and transient kinetic data revealed a mechanism in which the two enzymatic reactions are coupled by an activation ...
Becker, Donald F. +4 more
core +3 more sources
Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics [PDF]
, 2015 Methanogenic and methanotrophic archaea play important roles in the global flux of methane. Culture-independent approaches are providing deeper insight into the diversity and evolution of methane-metabolizing microorganisms, but, until now, no compelling
Chadwick, Grayson L. +6 more
core +1 more source
Pest Management Science, 2016 AbstractBACKGROUNDDual‐target inhibitors may contribute to the management of herbicide‐resistant weeds and avoid or delay the selection of resistant biotypes. Some aminobisphosphonates inhibit the activity of both glutamine synthetase and δ1‐pyrroline‐5‐carboxylate (P5C) reductase in vitro, but the relevance of the latter in vivo has yet to be proven ...
GIBERTI, Samuele +5 more
openaire +3 more sources
P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma [PDF]
, 2019 The present study aimed to investigate the expression of pyrroline-5-carboxylate reductase 1 (P5CR1) protein in lung adenocarcinoma and paracancerous tissues and to explore the effect of silencing the encoding gene PYCR1 on the proliferation, migration ...
Aiyou Mao +3 more
core +1 more source
Structural and kinetic studies of proline metabolic enzyme inhibitors and inactivators [PDF]
Structural and kinetic studies of the proline metabolic enzymes proline dehydrogenase (PRODH), [delta]1-pyrroline-5-carboxylate reductase (PYCR), and aldehyde dehydrogenase 4A1 (ALDH4A1) are presented in this thesis.
Bogner, Alexandra Nicole
core
Pyrroline-5-carboxylate reductase 1 (PYCR1) [PDF]
Science-Business eXchange, 2009 openaire +1 more source