Results 241 to 250 of about 64,563 (283)

Assay of Insulin Mediator Activity with Soluble Pyruvate Dehydrogenase Phosphatase

Endocrinology, 1985
The putative mediator of intracellular insulin action has been assayed quantitatively by its ability to increase the activity of solubilized pyruvate dehydrogenase (PDH) phosphatase. Conversion of soluble beef heart PDH b to PDH a by PDH phosphatase increased when incubation was carried out in the presence of a crude insulin mediator fraction generated
J D, Newman, J M, Armstrong, J, Bornstein   +2 more
openaire   +2 more sources

Starvation reduces pyruvate dehydrogenase phosphate phosphatase activity in rat kidney

Molecular and Cellular Biochemistry, 1995
Pyruvate dehydrogenase complex (PDC) from rat kidney or pig heart previously inactivated by phosphorylation (PDHP) was activated in vitro by PDHP phosphatase from kidneys of starved or fed rats. Starvation for 48 h of the rats from which the PDC was prepared led to a decrease in the rate of activation of PDC at early time periods (< 2 min ...
B N, Cockburn, H G, Coore
openaire   +2 more sources

Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms

2001
The mammalian pyruvate dehydrogenase complex (PDC) plays central and strategic roles in the control of the use of glucose-linked substrates as sources of oxidative energy or as precursors in the biosynthesis of fatty acids. The activity of this mitochondrial complex is regulated by the continuous operation of competing pyruvate dehydrogenase kinase ...
T E, Roche, J C, Baker, X, Yan, Y, Hiromasa, X, Gong, T, Peng, J, Dong, A, Turkan, S A, Kasten   +8 more
openaire   +2 more sources

Identification of a canine model of pyruvate dehydrogenase phosphatase 1 deficiency

Molecular Genetics and Metabolism, 2006
Exercise intolerance syndromes are well known to be associated with inborn errors of metabolism affecting glycolysis (phosphorylase and phosphofructokinase deficiency) and fatty acid oxidation (palmitoyl carnitine transferase deficiency). We have identified a canine model for profound exercise intolerance caused by a deficit in PDP1 (EC 3.1.3.43), the ...
Jessie M, Cameron, Mary C, Maj, Valeriy, Levandovskiy, Neviana, MacKay, G Diane, Shelton, Brian H, Robinson   +5 more
openaire   +2 more sources

Pyruvate dehydrogenase phosphatase deficiency: Orphan disease or an under-diagnosed condition?

Molecular and Cellular Endocrinology, 2006
Pyruvate dehydrogenase phosphatase (PDP) is an enzyme which regulates the activity of the pyruvate dehydrogenase complex (PDHc). In the past, PDHc deficiency has been attributed to mutations in the complex itself and the regulatory enzymes have not been considered.
M C, Maj, J M, Cameron, B H, Robinson
openaire   +2 more sources

Decrease of pyruvate dehydrogenase phosphatase activity in patients with congenital lactic acidemia

Clinica Chimica Acta, 1992
We developed an assay method for pyruvate dehydrogenase phosphatase activity using [1-14C]pyruvate and measured pyruvate dehydrogenase phosphatase activity in cultured skin fibroblasts from three patients with congenital lactic acidemia due to a defect in activation of the pyruvate dehydrogenase complex.
M, Ito, H, Kobashi, E, Naito, T, Saijo, E, Takeda, A H, Huq, Y, Kuroda   +6 more
openaire   +2 more sources

Pyruvate dehydrogenase phosphatase 1 (PDP1) null mutation produces a lethal infantile phenotype

Human Genetics, 2009
Pyruvate dehydrogenase phosphatase deficiency has previously only been confirmed at the molecular level in two brothers and two breeds of dog with exercise intolerance. A female patient, who died at 6 months, presented with lactic acidemia in the neonatal period with serum lactate levels ranging from 2.5 to 17 mM. Failure of dichloroacetate to activate
J M, Cameron, M, Maj, V, Levandovskiy, C P, Barnett, S, Blaser, N, Mackay, J, Raiman, A, Feigenbaum, A, Schulze, B H, Robinson   +9 more
openaire   +2 more sources

Purification and properties of pyruvate dehydrogenase phosphatase from bovine heart and kidney

Biochemistry, 1982
Pyruvate dehydrogenase phosphatase was purified to apparent homogeneity from bovine heart and kidney mitochondria. The phosphatase has a sedimentation coefficient (S20,w) of about 7.4 S and a molecular weight (Mr) of about 150 000 as determined by sedimentation equilibrium and by gel-permeation chromatography.
W M, Teague, F H, Pettit, T L, Wu, S R, Silberman, L J, Reed   +4 more
openaire   +2 more sources

One-Step Purification of the Recombinant Catalytic Subunit of Pyruvate Dehydrogenase Phosphatase

Protein Expression and Purification, 2000
A facile one-step affinity chromatographic purification of the recombinant catalytic subunit (PDPc) of bovine pyruvate dehydrogenase phosphatase (PDP) to near homogeneity is described. PDPc binds in the presence of Ca(2+) to the inner lipoyl domain (L2) of the dihydrolipoamide acetyltransferase component (E2) of the mammalian pyruvate dehydrogenase ...
W, Soo Choi, J, Yan, D B, McCarthy, S, Hee Park, L J, Reed   +4 more
openaire   +2 more sources

A model for the spatial location of pyruvate dehydrogenase phosphatase in mammalian pyruvate dehydrogenase complex.

Biochemistry. Biokhimiia, 1999
Recent experimental findings on the structural--functional features of pyruvate dehydrogenase phosphatase (PDP) isolated from various sources are compared. Two alternative mechanisms (a and b) of dephosphorylation of the E1 component in the pyruvate dehydrogenase complex (PDC) are discussed: a) the reaction occurs as a result of stochastic collisions ...
G L, Ermakov, B N, Goldstein
openaire   +1 more source