Results 121 to 130 of about 5,809 (168)

The combined QF-PCR and cytogenetic approach in prenatal diagnosis

Molecular Biology Reports, 2014
In this study, the importance of quantitative fluorescence polymerase chain reaction (QF-PCR) aneuploidy diagnosis test which provides earlier and easier results were discussed. The cell cultures and DNA isolations were performed on 100 amniotic fluids.
Tekcan, Akin, Tural, Sengul, Elbistan, Mehmet, Kara, Nurten, Guven, Davut, Kocak, Idris   +5 more
openaire   +2 more sources

The consanguinity effect on QF-PCR diagnosis of autosomal anomalies

Prenatal Diagnosis, 2006
Quantitative Fluorescent PCR (QF-PCR) is a simpler and faster method of detecting common chromosomal abnormalities when compared to cytogenetic analysis. The aim of our study is to investigate the applicability of this methodology in a population where consanguineous marriages are common and to estimate the heterozygous frequency of the PCR markers ...
Michel B, Choueiri, Nadine J, Makhoul, Tony G, Zreik, Farid, Mattar, Abdallah M, Adra, Raymond, Eid, Adnan M, Mroueh, Pierre A, Zalloua   +7 more
openaire   +3 more sources

Chromosomal abnormality diagnosis of male infertility by QF-PCR

Hereditas (Beijing), 2011
To assess the clinical practice of quantitative fluorescence PCR (QF-PCR) in genetic diagnosis of male infertility patients, 78 nonobstructive male infertility patients were pooled for semen routine screening and sexual hormone determination; QF-PCR was applied to detect the polymorphic short tandem repeat (STR) and specific sequence tagged site (STS ...
Man-Long QI, Yuan-Yuan ZHANG, Xiao-Liang LIU, Rong HE, Yan-Yan ZHAO   +4 more
openaire   +3 more sources

Prenatal Detection of Isochromosome 21 by QF-PCR

Fetal Diagnosis and Therapy, 2008
<i>Objective:</i> To compare rapid aneuploidy diagnostic tests with traditional karyotyping in the prenatal detection of Down syndrome due to isochromosome 21. <i>Methods:</i> Quantitative fluorescence PCR (QF-PCR) and fluorescent in situ hybridization (FISH) for chromosomes 13, 18, 21, X and Y were performed on uncultured ...
Leena Gole, Ng Bee Lian, Ng Poh Lian, Mary Rauff, Arijit Biswas, Mahesh Choolani   +5 more
openaire   +1 more source

A case of 48,XXYY syndrome detected prenatally by QF-PCR

The Journal of Maternal-Fetal & Neonatal Medicine, 2009
We report on the prenatal diagnosis and genetic analysis of a 48,XXYY fetus. A 28-year-old woman was referred for amniocentesis at 18 weeks' gestation because of advanced paternal age. Quantitative-fluorescence polymerase chain reaction (QF-PCR) with small tandem repeat (STR) markers rapidly detected the sex chromosome polysomy from amniotic fluid ...
Qiu-Shi, Zhang, Dong-Zhi, Li
openaire   +2 more sources

Validation of QF-PCR for prenatal aneuploidy screening in the United States

Prenatal Diagnosis, 2006
QF-PCR is an inexpensive and reliable method for aneuploidy screening; however, despite its obvious advantages, it is not in routine use in the United States. Our objective in the present study was to validate QF-PCR as a means for prenatal aneuploidy screening in our institution.A QF-PCR assay using 15 primer pairs located on chromosomes 13, 18, 21 X ...
Lucia, Brown, Melissa, Abigania, Dorothy, Warburton, Stephen, Brown   +3 more
openaire   +2 more sources

The role of the QF-PCR method in the detection of frequent aneuploidies

Nauchno-prakticheskii zhurnal «Medicinskaia genetika, 2023
Анеуплоидия – это изменение числа гомологичных хромосом в диплоидном наборе в клетке. Наиболее распространенными анеуплоидиями, совместимыми с живорождением, являются анеуплоидии по хромосомам 13, 18, 21, X и Y. В настоящее время для диагностики частых анеуплоидий возможно использование быстрого, надежного и недорогого метода количественной ...
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Use of a DNA Method, QF-PCR, in the Prenatal Diagnosis of Fetal Aneuploidies

Journal of Obstetrics and Gynaecology Canada, 2011
To provide Canadian health care providers with current information on the use of quantitative fluorescent polymerase chain reaction (QF-PCR) or equivalent technology in the prenatal diagnosis of fetal chromosomal abnormalities.Over the last few decades, prenatal diagnosis of fetal chromosomal abnormalities has relied on conventional cytogenetic ...
Sylvie Langlois, Alessandra Duncan, R. Douglas Wilson, François Audibert, Jo-Ann Brock, June Carroll, Lola Cartier, Valérie A. Désilets, Alain Gagnon, Jo-Ann Johnson, Sylvie Langlois, Lynn Murphy-Kaulbeck, Nanette Okun, Melanie Pastuck, Sylvie Langlois, David Chitayat, Isabelle DeBie, Suzanne Demczuk, Valérie A. Désilets, Alessandra Duncan, Michael T. Geraghty, Janet Marcadier, Tanya N. Nelson, Vicky Siu, David Skidmore   +24 more
openaire   +2 more sources

Maternal cell contamination of prenatal samples assessed by QF-PCR genotyping

Prenatal Diagnosis, 2005
To establish the genotype of cultured cells from a cohort of amniotic fluid and chorionic villus samples, and compare this genotype with that obtained from uncultured material from the same sample, in order to assess the frequency and significance of maternal cell contamination of prenatal samples.Quantitative fluorescence-polymerase chain reaction (QF-
Taita, Stojilkovic-Mikic, Kathy, Mann, Zoe, Docherty, Caroline, Mackie Ogilvie   +3 more
openaire   +2 more sources

QF‐PCR‐based prenatal detection of aneuploidy in a southeast Asian population

Prenatal Diagnosis, 2004
AbstractObjectivesWe have investigated the efficacy of using quantitative fluorescent polymerase chain reaction (QF‐PCR) for the prenatal recognition of aneuploidy in chromosomes 13, 18, 21, X and Y. A total of 1115 samples, from mainly southeast Asian patients, were analysed and compared in a blind trial to the results previously obtained ...
R, Quaife, L F, Wong, S Y, Tan, W Y, Chua, S S, Lim, C J N, Hammersley, H L, Yeo   +6 more
openaire   +2 more sources