Results 131 to 140 of about 2,590,147 (263)

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Emerging quality-by-design optimized HPLC method for the concurrent determination of cefixime and ornidazole: a multi-criteria green and blue environmental footprinting. [PDF]

Sci Rep
Mostafa YE, Elsebaei F, Metwally ME.
europepmc   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

A Survey on the Implementation of Analytical Quality by Design in Method Development in the Pharmaceutical Industries of Southeast Brazil: Challenges and Opportunities. [PDF]

Ther Innov Regul Sci
Claro BC, Condado EF, Prado LD.
europepmc   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Green Synthesis and Quality-by-Design Optimization of <i>Dacryodes edulis</i>-Derived Silver Nanoparticles with Broad-Spectrum Antiviral and Antimicrobial Activity. [PDF]

Molecules
Xulu JH, Tembu VJ, Moeno S, Tsakem B, Thibane VS, Witika BA, Siwe Noundou X.   +6 more
europepmc   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Quality by Design Approach for Hot-Melt Extrusion Coupled Fused Deposition Modeling (HME-FDM) 3D Printing: A Systematic Review. [PDF]

Pharmaceutics
Arany P, Papp Á, Nemes D, Fehér P, Ujhelyi Z, Bácskay I.   +5 more
europepmc   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Protocol for applying an enhanced quality-by-design program across the translational science spectrum. [PDF]

J Clin Transl Sci
Orechwa AZ, Aguilar L, Castiel M, Huang C, Dzekov J, Maccalla NMG, Mack W, Mele Z, Moses K, Patino-Sutton C, Shah S, Towfighi A, Buchanan T.   +12 more
europepmc   +1 more source