Laser-induced fluorescence quenching of red fluorescent dyes with green excitation: avoiding artifacts in PIE-FRET and FCCS analysis [PDF]
Multicolor excitation is at the core of many fluorescence spectroscopy techniques such as PIE-FRET, ALEX-FRET, or FCCS. However, the influence of the multiple laser excitations on the dye photophysics is often overlooked. Here, we show that green laser pulses can surprisingly quench the fluorescence of common red dyes Alexa Fluor 647 and Atto 647N ...
arxiv +1 more source
A new, simple, selective, sensitive, fast, economical, and reliable fluorescence quenching method for the quantitation of valsartan was investigated using basic fuchsin act as a fluorometric dye.
Layth I. Abd Ali
doaj +1 more source
Fluorescence quenching near small metal nanoparticles
We develop a microscopic model for fluorescence of a molecule (or semiconductor quantum dot) near a small metal nanoparticle. When a molecule is situated close to metal surface, its fluorescence is quenched due to energy transfer to the metal. We perform
Pustovit, Vitaliy N.+1 more
core +1 more source
Solvatochromism of (-)-Epigallocatechin 3-O-Gallate (EGCG) Fluorescence: Dependence on Solvent Protonicity [PDF]
Health benefits of EGCG are well established; however, the mechanisms of EGCG action are not completely understood. In our previous study we discovered solvatochromism of EGCG fluorescence and described the dependence of fluorescence maxima on solvent polarity. We also noted that fluorescence intensity (FI) depends on solvent.
arxiv
The interaction between the anti-HIV drug Elvitegravir (EVG) and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy and UV-visible absorption spectra. The mechanism for quenching the fluorescence of BSA by EVG is discussed.
Ali Saber Abdelhameed
doaj +1 more source
In the current study, the interaction of minocycline hydrochloride (MC) and trypsin (TRP) was studied using fluorescence spectroscopy, synchronous fluorescence spectroscopy, three-dimensional fluorescence spectroscopy, UV-Vis spectroscopy, and molecular ...
Xiaoxia Wang+6 more
doaj +1 more source
Fluorescence signalling of the transition metal ions: design strategy based on the choice of the fluorophore component [PDF]
Transition metal ions are notorious for their fluorescence quenching abilities. In this paper, we discuss the design strategies for the development of efficientoff-on fluorescence signalling systems for the transition metal ions.
Banthia, S.+2 more
core +1 more source
Fluorescence quenching in plasmonic dimers due to electron tunneling [PDF]
Plasmonic nanoparticles provide an ideal environment for the enhancement of fluorescent emission. On the one hand, they locally amplify the electromagnetic fields, increasing the emitter excitation rate, and on the other hand, they provide a high local density of states that accelerates spontaneous emission. However, when the emitter is placed in close
arxiv
Anthracene Fluorescence Quenching by a Tetrakis (Ketocarboxamide) Cavitand
Quenching of both fluorescence lifetime and fluorescence intensity of anthracene was investigated in the presence of a newly derived tetrakis (ketocarboxamide) cavitand at various concentrations.
Tibor Zoltan Janosi+5 more
doaj +1 more source
Tryptophan Fluorescence Quenching in β-Lactam-Interacting Proteins Is Modulated by the Structure of Intermediates and Final Products of the Acylation Reaction [PDF]
In most bacteria, β-lactam antibiotics inhibit the last cross-linking step of peptidoglycan synthesis by acylation of the active-site Ser of d,d-transpeptidases belonging to the penicillin-binding protein (PBP) family.
A. Dupuis+10 more
core +3 more sources