Results 81 to 90 of about 1,191,294 (396)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

The photoswitchable cannabinoid azo‐HU308 enables optical control of Ca2+ dynamics in INS‐1 β‐cells via off‐target effects on TRPC channels

FEBS Open Bio, EarlyView.
Light activation of the photoswitchable cannabinoid ligand azo‐HU308 triggers Ca2+ influx in pancreatic β‐cells through TRPC channels, independent of CB2 cannabinoid receptors. This reveals a non‐GPCR pathway for cannabinoid modulation of β‐cell Ca2+ dynamics and establishes azo‐HU308 as an optical tool to study cannabinoid signaling through TRP ...
Alexander E. G. Viray, James A. Frank
wiley   +1 more source

Comparative Studies on the Interaction of Cochinchinenin A and Loureirin B with Bovine Serum Albumin

Journal of Spectroscopy, 2013
This paper describes the simple, sensitive, and effective spectrophotometric methods based on ultraviolet, fluorescence and circular dichroism for revealing the interactional mechanism of Cochinchinenin A (CA) and Loureirin B (LB) with bovine serum ...
Tianming Yang, Hao Zhang, Haiyan Fu, Yuanbin She, Can Huang, Yan Hu, Liping Wang   +6 more
doaj   +1 more source

Comparison of Anionic, Cationic and Nonionic Surfactants as Dispersing Agents for Graphene Based on the Fluorescence of Riboflavin

Nanomaterials, 2017
Fluorescence quenching is a valuable tool to gain insight about dynamic changes of fluorophores in complex systems. Graphene (G), a single-layered 2D nanomaterial with unique properties, was dispersed in surfactant aqueous solutions of different nature ...
Rocío Mateos, Soledad Vera, Mercedes Valiente, Ana María Díez-Pascual, María Paz San Andrés   +4 more
doaj   +1 more source

Studies on the changes in protein fluorescence and enzymic activity of aspartate aminotransferase on binding of pyridoxal 5'-Phosphate [PDF]

, 1974
1. The a and ,B subforms of aspartate aminotransferase were purified from pig heart. 2. The a subform contained 2mol of pyridoxal 5'-phosphate. The apo-(a subform) could be fully reactived by combination with 2mol of cofactor. 3.
Evans, RW, Holbrook, J
core   +2 more sources

Fluorescence Quenching of Xanthene Dyes during Amide Bond Formation using DMTMM [PDF]

, 2022
Kristen Patterson, Misael A. Romero-Reyes, Jennifer M. Heemstra   +2 more
openalex   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

FEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava, Anoop Saraya, Deepak Gunjan, Rinu Sharma   +3 more
wiley   +1 more source

Synthesis and photophysical studies on 5-ethoxycarbonyl-4-cinnamyl-6-methyl-3,4-dihydropyrimidine-2(1H)-one in various solvents

Bulletin of the Chemical Society of Ethiopia, 2009
The 5-ethoxycarbonyl-4-cinnamyl-6-methyl-3,4-dihydropyrimidine-2(1H)-one (DHPM) was synthesized via Biginelli reaction and was characterized by NMR, IR, UV−Visible absorption and fluorescence spectroscopy.
Sheela N. Lad, Umesh S. Mote, Swaminath L. Bhattar, Prashant V. Anbhule, Shivajirao R. Patil   +4 more
doaj  

Quenching dynamics in CdSe nanoparticles: surface-induced defects upon dilution [PDF]

, 2012
International audienceWe have analyzed the decays of the fluorescence of colloidal CdSe quantum dots (QDs) suspensions during dilution and titration by the ligands.
Bardet, Michel, Baxter, Paul N W, Chandezon, Frédéric, Fiore, Angela, Gromova, Marina, Hartmann, Lucia, Julien-Rabant, Carine, Kumar, Abhishek, Pansu, Robert,, Reiss, Peter, Welker, Matthias   +10 more
core   +2 more sources

β‐Catenin/c‐Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations

Advanced Biology, Volume 9, Issue 3, March 2025.
Ammonia, detoxified by the liver into urea and glutamine, impacts autophagy differently at varying levels. Low ammonia activates autophagy via c‐Myc and β‐catenin, while high levels suppress it. Using Huh7 cells and Spf‐ash mice, c‐Myc's role in cytoprotective autophagy is revealed, offering insights into hyperammonemia and potential therapeutic ...
S. Sergio, B. Spedicato, G. Corallo, A. Inguscio, M. Greco, D. Musarò, D. Vergara, A. F. Muro, G. De Sabbata, L. R. Soria, N. Brunetti Pierri, M. Maffia   +11 more
wiley   +1 more source