Results 171 to 180 of about 3,104,231 (310)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

R: una herramienta poco difundida y muy útil para la investigación clínica R: a not much spread and very useful tool for clinical research

Revista Cubana de Investigaciones Biomédicas, 2010
Con el fin de estimular el uso de R en la investigación clínica y a partir de la experiencia con este software para el procesamiento y análisis de datos, se muestran algunas de sus características y las principales ventajas que ofrece su utilización en ...
MayelÍn Mirabal Sosa, Maytee Robaina García, Rolando Uranga Piña   +2 more
doaj  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Intervention meta-analysis: application and practice using R software. [PDF]

Epidemiol Health, 2019
Shim SR, Kim SJ.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Network meta-analysis: application and practice using R software. [PDF]

Epidemiol Health, 2019
Shim SR, Kim SJ, Lee J, Rücker G.
europepmc   +1 more source

Does software piracy always represent consumer misbehaviour?

, 2011
This study aims to explore whether or not software piracy is perceived as consumer misbehaviour in Libya. Both qualitative and quantitative methods have been used; data were collected by interviewing 10 marketers and through a questionnaire surveying 219
Whysall, P, Abdelhadi, A., Abdelhadi, A, Foster, C, Whysall, P., Foster, Carley   +5 more
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Protocol to detect dilution cycles in chemostat experiments and estimate growth rate slopes with linear modeling with R software chemostat_regression. [PDF]

STAR Protoc
Koehler SI, Pentz JT, Middlebrook EA, Hovde BT, Hanschen ER.   +4 more
europepmc   +1 more source