Results 121 to 130 of about 174,052,608 (250)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Assessing the Accuracy and Reliability of the Monitored Augmented Rehabilitation System for Measuring Shoulder and Elbow Range of Motion

Sensors
Accurate range of motion (ROM) assessment is essential for evaluating musculoskeletal function and guiding rehabilitation, particularly in pediatric populations.
Samuel T. Lauman, Lindsey J. Patton, Pauline Chen, Shreya Ravi, Stephen J. Kimatian, Sarah E. Rebstock   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Effect of Pilates Exercise on Range of Motion and Edema of Upper Limb in Mastectomy Side

Journal of Ardabil University of Medical Sciences, 2013
Background & objectives : The surgery of breast cancer like any other surgeries may bring about some problems and complications, which the knowledge of these problems may be an effective way for prevention or dealing with the complications. The motor and
Maryam Ghorbani, Yahia Sokhangouei, Heidar Sadeghi   +2 more
doaj  

A randomized controlled trial with extended long-term follow-up: Quality of cervical spine motion after anterior cervical discectomy (ACD) or anterior cervical discectomy with arthroplasty (ACDA)

Brain and Spine
Introduction: In previous research, a consistent sequence of segmental contributions during dynamic extension X-rays of the cervical spine was observed in 80–90% of healthy participants.
Valérie N.E. Schuermans, Anouk Y.J.M. Smeets, Inez Curfs, Henk van Santbrink, Toon F.M. Boselie   +4 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

The influence of contraction duration in muscle energy technique applied to the atlanto-axial joint [PDF]

, 2004
Background: Muscle Energy Technique (MET) has been advocated for the treatment of restricted range of motion in the upper neck. There is little evidence, however, to support the effectiveness of MET to increase motion in the cervical spine, or determine ...
Fryer, Gary, Ruszkowski, Wojtek
core  

Reliability and Validity of the Single-Camera Markerless Motion Capture System for Measuring Shoulder Range of Motion in Healthy Individuals and Patients with Adhesive Capsulitis: A Single-Center Study

Sensors
Assessing shoulder joint range of motion (ROM) is essential for diagnosing musculoskeletal disorders and optimizing treatments. This single-center pilot study evaluated the reliability and validity of iBalance, a single-camera markerless motion capture ...
Suji Lee, Unhyung Lee, Yohwan Kim, Seungjin Noh, Hungu Lee, Seunghoon Lee   +5 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source