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RAPD analysis of Yersinia enterocolitica
Letters in Applied Microbiology, 1994A total of 87 isolates of Yersinia enterocolitica were examined with randomly amplified polymorphic DNA (RAPD) by use of three different primers. Based on the RAPD profiles, the strains could be divided into three major groups: (1) the pathogenic American serotypes, O:8, O:13ab, O:20 and O:21; (2) the pathogenic European serotypes, O:3, O:5,27 and O:9;
Rasmussen, H. N. +2 more
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RAPD problems in phylogenetics
Zoologica Scripta, 1996This paper is intended to clarify some of the questions related with the application of RAPD for phylogenetic reconstruction purposes. Using different specimens of mammals selected across various taxonomic levels, we assessed the validity of RAPD to recover a known phylogeny, using four distance coefficients (simple matching, Russell & Rao, Jaccard,
François-Joseph Lapointe +1 more
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1997
Cloning of RAPD markers is a valuable technique for the study and utilization of RAPD amplification products. It can contribute to the characterization of a DNA region that is species- or group-specific, allowing the construction of probes and oligonucleotides to be used for the detection of microorganisms (see Chap. XXV).
E. Mori, FANI, RENATO
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Cloning of RAPD markers is a valuable technique for the study and utilization of RAPD amplification products. It can contribute to the characterization of a DNA region that is species- or group-specific, allowing the construction of probes and oligonucleotides to be used for the detection of microorganisms (see Chap. XXV).
E. Mori, FANI, RENATO
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RAPD identification of microsatellites in Daphnia
Molecular Ecology, 1996AbstractSimple sequence repeats (SSRs, or microsatellites) have been constantly gaining importance as single‐locus DNA markers in population genetics and behavioural ecology. We tested a PCR‐based strategy for finding microsatellite loci in anonymous genomes, which avoids genomic library construction and screening, and the need for larger amounts of ...
Bruno Streit +5 more
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1997
Although the random amplified polymorphic DNA (RAPD) methodology, described by Williams et al. (1990) and Welsh and McClelland (1990), has been extensively used for many purposes, very little is known about the nucleotide sequence of RAPD markers and the primer binding sites within the target genome.
E. Mori, FANI, RENATO
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Although the random amplified polymorphic DNA (RAPD) methodology, described by Williams et al. (1990) and Welsh and McClelland (1990), has been extensively used for many purposes, very little is known about the nucleotide sequence of RAPD markers and the primer binding sites within the target genome.
E. Mori, FANI, RENATO
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The use of RAPDs for the analysis of parasites
1993There is a lack of sequence information concerning polymorphic loci in parasite genomes. Thus, the use of arbitrary PCR primers under low temperature annealing conditions to generate random amplified polymorphic DNAs (RAPDs) represents an important approach to the study of the structure of parasite populations, their genetic variation as well as ...
Mário Steindel +5 more
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Impact of scoring error and reproducibility RAPD data on RAPD based estimates of genetic distance
Theoretical and Applied Genetics, 1995RAPD band reproducibility and scoring error were evaluated for RAPDs generated by 50 RAPD primers among ten snap bean (Phaseolus vulgaris L.) genotypes. Genetic distances based on different sets of RAPD bands were compared to evaluate the impact of scoring error, reproducibility, and differences in relative amplification strength on the reproducibility
Paul W. Skroch, J. Nienhuis
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2002
Randomly amplified polymorphic DNA (RAPD)- polymerase chain reaction (PCR) is a simple and inexpensive tool enabling the study of genetic variation at population level. It can also be applied at the species or subgeneric level to examine phylogenetic questions (e.g., Yoon and Bae 1995, Altomare et al 1997, Gandeboeuf et al. 1997).
Imke Schmitt, H. Thorsten Lumbsch
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Randomly amplified polymorphic DNA (RAPD)- polymerase chain reaction (PCR) is a simple and inexpensive tool enabling the study of genetic variation at population level. It can also be applied at the species or subgeneric level to examine phylogenetic questions (e.g., Yoon and Bae 1995, Altomare et al 1997, Gandeboeuf et al. 1997).
Imke Schmitt, H. Thorsten Lumbsch
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Competition as a source of errors in RAPD analysis
TAG Theoretical and Applied Genetics, 1996We have used artificial 1∶1 DNA mixtures of all pairwise combinations of four doubled haploid Brassica napus lines to test the ability of RAPDs to function as reliable dominant genetic markers. In situations where a specific RAPD band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in
Christer Halldén +4 more
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RAPD Fingerprinting of Chlorobium Strains
Systematic and Applied Microbiology, 1998Summary In vitro amplification of genomic DNA fragments with single primers of arbitrary sequence was used as a rapid and sensitive method to obtain fingerprints of fourteen strains belonging to five Chlorobium species. Each strain showed a distinctive pattern of bands that permitted its unequivocal identification when the primer gyr lower was used ...
Isabel Esteve +3 more
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