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DNA sequences of RAPD fragments in artiodactyls [PDF]
Genome, 1996 A bovine RAPD profile, generated by a 10-mer primer, was analysed by sequencing the major fragments. Three of four different fragments showed homologies to previously characterized mammalian sequences. One was 61–66% identical to LINE sequences and another was 78.5% identical to a human chromosome 2 sequence tagged site.
Sirkka-Liisa Varvio +2 more
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An evaluation of RAPD fragment reproducibility and nature
Molecular Ecology, 1998Random amplified polymorphic DNA (RAPD) fragment reproducibility was assayed in three animal species: red deer (Cervus elaphus), wild boar (Sus scrofa) and fruit fly (Drosophila melanogaster). Ten 10‐mer primers (Operon) were tested in two replicate reactions per individual under different stringency conditions (annealing temperatures of 35 °C or 45 °C)
Trinidad Pérez +2 more
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RAPD in the analysis of isolates of Entamoeba histolytica
Acta Tropica, 2000Genetic variability in Entamoeba histolytica was analyzed by random amplified polymorphic DNA (RAPD) using ten arbitrary primers. Due to intrinsic characteristics of the RAPD technique only axenic samples were analyzed. since the presence of any microorganism in the cultures interfered in the DNA profile by generating RAPDs not pertaining to E ...
Edward F. Silva +4 more
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Inheritance of RAPDs in F1 hybrids of corn
Theoretical and Applied Genetics, 1993Random amplified polymorphic DNA (RAPD) markers were analyzed in materials from a partial diallel, including 16 corn F1 hybrids (with five reciprocals) and their five parental inbreds. Using 21 primers, we scored a total of 140 different fragments for their presence/absence and intensity variation, where appropriate.
T. Helentjaris, M. Heun
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Analysis of plum cultivars with RAPD markers
Journal of Horticultural Science, 1997SummaryHexaploid and diploid plum cultivars from the collection of Aula Dei (Zaragoza, Spain) were examined by means of RAPDs (random amplified polymorphic DNAs). A high degree of polymorphism was detected and it was possible to distinguish all 31 genotypes by combining the RAPDs patterns of three primers: AD16, AD14 and B1. Genotype-specific RAPDs and
Ortiz, A. +3 more
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Rapd profiling of oxyopidae spiders
Indian Journal of Entomology, 2019Random amplified polymorphic DNA(RAPD) Polymerase Chain Reaction (PCR) was used to characterize six spiders belonging to the family Oxyopidae. The aim was to achieve genetic marker to determine the genetic variations of these species. The species studied includes: Oxyopes lineatipes (C L Koch, 1847), O. javanus (Thorell, 1887), O. shweta (Tikader, 1970)
S. Arunkumar, T.N. Ramakrishnaiah
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Genetic Analysis with RAPD Markers
1994For many years the principles of genetics have been applied to crop variety improvement with great success. Several crop species, notably corn, wheat, and tomato, have been used as model genetic systems because of their central importance to food production. Until recently, virtually all progress in both breeding and model genetic systems has relied on
Michael K. Hanafey +2 more
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Optimization of RAPD Fingerprinting
1997RAPD fingerprinting has been extensively used to detect DNA sequence polymorphism in many plant, bacterial and fungal species (Williams et al. 1990; Kresovich et al. 1992; Cobb and Clarkson 1993). In addition, tightly linked RAPD markers have been used to detect breeding lines containing specific gene blocks facilitating marker-based selection and ...
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Arbitrarily Primed PCR and RAPDs
1997The arbitrarily primed polymerase chain reaction (AP-PCR) is a PCR-based DNA fingerprinting technique using primers whose nucleotide sequence is arbitrarily chosen (Welsh and McClelland 1990; Williams et al. 1990). This method has also been called random amplified polymorphic DNA (RAPD). Initial cycles of the reaction are performed under low stringency
R. Arribas +4 more
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