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Competition as a source of errors in RAPD analysis
TAG Theoretical and Applied Genetics, 1996We have used artificial 1∶1 DNA mixtures of all pairwise combinations of four doubled haploid Brassica napus lines to test the ability of RAPDs to function as reliable dominant genetic markers. In situations where a specific RAPD band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in
Christer Halldén +4 more
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RAPD Fingerprinting of Chlorobium Strains
Systematic and Applied Microbiology, 1998Summary In vitro amplification of genomic DNA fragments with single primers of arbitrary sequence was used as a rapid and sensitive method to obtain fingerprints of fourteen strains belonging to five Chlorobium species. Each strain showed a distinctive pattern of bands that permitted its unequivocal identification when the primer gyr lower was used ...
Isabel Esteve +3 more
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RAPD Analysis of Maize Somaclones
Russian Journal of Genetics, 2001The genetic difference between maize line A188 and A188-derived somaclones was assessed via analysis of randomly amplified polymorphic DNA (RAPD). In total, 17 decanucleotide primers used each allowed amplification of 2–17 fragments ranging 200–2000 bp.
S. A. Gostimskij +5 more
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RAPD in the analysis of isolates of Entamoeba histolytica
Acta Tropica, 2000Genetic variability in Entamoeba histolytica was analyzed by random amplified polymorphic DNA (RAPD) using ten arbitrary primers. Due to intrinsic characteristics of the RAPD technique only axenic samples were analyzed. since the presence of any microorganism in the cultures interfered in the DNA profile by generating RAPDs not pertaining to E ...
Edward F. Silva +4 more
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DNA sequences of RAPD fragments in artiodactyls [PDF]
Genome, 1996 A bovine RAPD profile, generated by a 10-mer primer, was analysed by sequencing the major fragments. Three of four different fragments showed homologies to previously characterized mammalian sequences. One was 61–66% identical to LINE sequences and another was 78.5% identical to a human chromosome 2 sequence tagged site.
Sirkka-Liisa Varvio +2 more
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An evaluation of RAPD fragment reproducibility and nature
Molecular Ecology, 1998Random amplified polymorphic DNA (RAPD) fragment reproducibility was assayed in three animal species: red deer (Cervus elaphus), wild boar (Sus scrofa) and fruit fly (Drosophila melanogaster). Ten 10‐mer primers (Operon) were tested in two replicate reactions per individual under different stringency conditions (annealing temperatures of 35 °C or 45 °C)
Trinidad Pérez +2 more
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Inheritance of RAPDs in F1 hybrids of corn
Theoretical and Applied Genetics, 1993Random amplified polymorphic DNA (RAPD) markers were analyzed in materials from a partial diallel, including 16 corn F1 hybrids (with five reciprocals) and their five parental inbreds. Using 21 primers, we scored a total of 140 different fragments for their presence/absence and intensity variation, where appropriate.
T. Helentjaris, M. Heun
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Rapd profiling of oxyopidae spiders
Indian Journal of Entomology, 2019Random amplified polymorphic DNA(RAPD) Polymerase Chain Reaction (PCR) was used to characterize six spiders belonging to the family Oxyopidae. The aim was to achieve genetic marker to determine the genetic variations of these species. The species studied includes: Oxyopes lineatipes (C L Koch, 1847), O. javanus (Thorell, 1887), O. shweta (Tikader, 1970)
S. Arunkumar, T.N. Ramakrishnaiah
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Analysis of plum cultivars with RAPD markers
Journal of Horticultural Science, 1997SummaryHexaploid and diploid plum cultivars from the collection of Aula Dei (Zaragoza, Spain) were examined by means of RAPDs (random amplified polymorphic DNAs). A high degree of polymorphism was detected and it was possible to distinguish all 31 genotypes by combining the RAPDs patterns of three primers: AD16, AD14 and B1. Genotype-specific RAPDs and
Ortiz, A. +3 more
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Genetic Analysis with RAPD Markers
1994For many years the principles of genetics have been applied to crop variety improvement with great success. Several crop species, notably corn, wheat, and tomato, have been used as model genetic systems because of their central importance to food production. Until recently, virtually all progress in both breeding and model genetic systems has relied on
Michael K. Hanafey +2 more
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