A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
A unified rodent atlas reveals the cellular complexity and evolutionary divergence of the dorsal vagal complex. [PDF]
Hes C +6 more
europepmc +1 more source
HSP70 governs permeability and mechanotransduction in primary human endothelial cells
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez +5 more
wiley +1 more source
Detection of Rat Lungworms in Invasive Mollusks, Georgia, USA, 2024. [PDF]
Achatz TJ +7 more
europepmc +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Rat Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Promote the Proliferation, Invasion, and Metastasis and Inhibit Apoptosis of Colorectal Cancer Stem Cells. [PDF]
Jing Y +8 more
europepmc +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Rat Glioma 101.8 Tissue Strain: Molecular and Morphological Features. [PDF]
Alekseeva AI +7 more
europepmc +1 more source

