SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Columbia suicide severity rating scale screen scores in adults with Chiari malformation Type 1. [PDF]
Labuda R +5 more
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Correction: Characteristics of Interaction Between Caregivers and Children with Chronic Diseases in Oral Medication‑Taking Situations: A Validation Study of the Interaction Rating Scale. [PDF]
Yasumoto T +4 more
europepmc +1 more source
Expert evaluation of mobile health apps for breast cancer management: a feature-based analysis using the Mobile Application Rating Scale (MARS) [PDF]
Ghazaleh Mohammadi Mogharab +5 more
openalex +1 more source
What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman +4 more
wiley +1 more source
Suicidal Ideation Among Patients With Breast Cancer: A Comparison of Patient Health Questionnaire-9 Item 9 and Columbia-Suicide Severity Rating Scale. [PDF]
Kim H, Park CHK.
europepmc +1 more source

