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Real-Time Polymerase Chain Reaction [PDF]

Springer Protocols Handbooks, 2020
: The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule.
Alangar Ishwara Bhat, Govind Pratap Rao
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Real‐Time Polymerase Chain Reaction

ChemBioChem, 2003
AbstractReal‐time PCR is the state‐of‐the‐art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real‐time PCR machines of different design is continuously increasing.
Alfred Pingoud, Jochen Wilhelm
openaire   +4 more sources

Real-time polymerase chain reaction

Resonance, 2008
Real Time PCR is a new advancement in the field of molecular diagnostics. The evolution of capabilities of Real Time PCR from traditional PCR has provided researchers an upper hand over the quantification and detection of specific DNA sequences. During the recent years, the areas of application of Real Time PCR have expanded exponentially.
Sarabjot Singh Pabla, Simarjot Pabla
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Real-Time Polymerase Chain Reaction

2010
Lut Overbergh, Anna-Paula Giulietti, Dirk Valckx, Chantal Mathieu   +3 more
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Detection of Castor Contamination by Real-Time Polymerase Chain Reaction [PDF]

Journal of Agricultural and Food Chemistry, 2006
Due to the potential for intentional contamination of food with crude preparations containing ricin, a real-time PCR method was developed for the detection of castor plant material in ground beef. One primer pair was identified and confirmed to be castor-specific and efficient for amplification of ricin in DNA extracts from castor or beef matrices.
Grace Q. Chen, John Mark Carter, Xiaohua He, David L. Brandon, Thomas A. McKeon   +4 more
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Electrochemical Real-Time Polymerase Chain Reaction

Journal of the American Chemical Society, 2006
In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon the advantages of the well-established fluorescence-based counterpart, such as short assay time (simultaneous target DNA amplification and detection).
Yeung, Siu Wai, Lee, Ming Hung Thomas, Hsing, I-Ming   +2 more
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Real-Time Polymerase Chain Reaction Detection Methods

Recent Patents on DNA & Gene Sequences, 2008
Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification.
Sherry A Dunbar, Douglas F. Whitman
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Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction.

Biotechnology and Bioengineering, 2005
Real-time polymerase chain reaction (PCR) is a highly sensitive method that can be used for the detection and quantification of microbial populations without cultivating them in anaerobic processes and environmental samples.
Youngseob Yu, Changsoo Lee, Jaai Kim, Seokhwan Hwang   +3 more
semanticscholar   +1 more source

Robust regression methods for real-time polymerase chain reaction

Analytical Biochemistry, 2015
Current real-time polymerase chain reaction (PCR) data analysis methods implement linear least squares regression methods for primer efficiency estimation based on standard curve dilution series. This method is sensitive to outliers that distort the outcome and are often ignored or removed by the end user.
Linos Vandekerckhove, K. Bosman, Ward De Spiegelaere, Jan De Neve, Wim Trypsteen, Monique Nijhuis, Olivier Thas, Olivier Thas   +7 more
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Analysis and Normalization of Real-Time Polymerase Chain Reaction (PCR) Experimental Data.

Cold Spring Harbor Protocols, 2018
In real-time polymerase chain reaction (PCR), also called quantitative real-time PCR [or simply quantitative PCR (qPCR)] or kinetic PCR, the amplification of DNA is monitored by the detection and quantitation of a fluorescent reporter signal, which ...
Michael R Green, J. Sambrook
semanticscholar   +1 more source