Results 341 to 350 of about 734,685 (383)

The Use of Real-Time Polymerase Chain Reaction in Thoracic Malignancies

Thoracic Surgery Clinics, 2006
Recent progress in the molecular analysis of NSCLC tumors and lymph node status will likely translate into a clearer understanding of the variables and predictors of tumor recurrence. This understanding may lead to more appropriate therapeutic decisions both in the operating room and in the clinic.
Jonathan D'Cunha, Michael A. Maddaus
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Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR)

Cold Spring Harbor Protocols, 2018
There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard polymerase chain reaction (PCR). In real-time PCR, it is necessary, however, to optimize the concentration of primers and probe and to perform a standard curve.
Michael R. Green, Joseph Sambrook
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Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction

2010
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform.
Ryan S. Robetorye, Hongxin Fan
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Detection of Plasmodium knowlesi by Real-Time Polymerase Chain Reaction

The American Journal of Tropical Medicine and Hygiene, 2009
We previously developed a real-time polymerase chain reaction (PCR) assay for detection of the four Plasmodium species that infect humans. Recent studies have shown that natural transmission of the simian parasite Plasmodium knowlesi to humans occurs frequently in Southeast Asia. We have expanded our PCR assay to include detection of P. knowlesi.
N. Esther Babady, Lynne M. Sloan, Jon E. Rosenblatt, Bobbi S. Pritt   +3 more
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Real-Time Polymerase Chain Reaction and Melting Curve Analysis

2006
Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of nucleic acid sequences and has become known as "real-time" PCR. Absolute quantification of initial template copy number can be obtained, although quantification relative to a control sample or second sequence is often adequate. Melting
Carl T. Wittwer, Robert J. Pryor
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Real-time polymerase chain reaction

2009
The invention and successful practice of the polymerase chain reaction (PCR) by Kary Mullis and colleagues in 1983 set the stage for a scientific revolution. PCR established a base technology from which many specific and diverse applications have grown.
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Real-Time Quantitative Polymerase Chain Reaction to Assess Gene Transfer

Human Gene Therapy, 1999
Accurate quantification of gene transfer (or gene correction) is a universal challenge in the field of gene therapy. In developing a clinical trial of lymphocyte gene therapy for Hunter syndrome (mucopolysaccharidosis type II), methods using Southern blot or automated DNA sequencing technology were employed, but found to be laborious and subject to ...
Dao Pan, Kathryn Becker, Chester B. Whitley   +2 more
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Reagent volume and plate bias in real-time polymerase chain reaction

Analytical Biochemistry, 2005
When optimal conditions are employed, real-time polymerase chain reaction (PCR) is a sensitive and accurate technique enabling the quantiWcation of low-copynumber transcripts [1]. However, as with conventional PCR, small variations in initial reaction conditions are ampliWed exponentially and can signiWcantly aVect results [2].
Errol M. Thomson, Renaud Vincent
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Polymerase Chain Reaction (PCR) and Real-Time PCR

2013
The PCR technique, developed over 3 decades ago, has revolutionized molecular biology. PCR applications encompass several fields, including genetics, molecular cloning, diagnosis of infectious and hereditary diseases, and forensic sciences. Improvements of the PCR technique have resulted in its expansion to include variants such as reverse ...
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Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.

Analytical Biochemistry, 1999
In this article we present validation of a real-time RT-PCR method to quantitate mRNA expression levels of atrial natriuretic peptide and c-fos in an in vitro model of cardiac hypertrophy.
J. Winer, Christopher Kwang S. Jung, Irene Shackel, P. Williams   +3 more
semanticscholar   +1 more source