Results 101 to 110 of about 1,797,163 (336)

Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction [PDF]

, 2005
Aim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.
Chen, Y, He, ML, Kung, HF, Li, Z, Lin, MC, Lu, GX, Peng, Y, Qian, WP, Shing, LK, Tan, YQ   +9 more
core   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Troubleshooting Real-Time PCR Screening for Leishmania infantum

Journal of Arthropod-Borne Diseases, 2018
Troubleshooting Real-Time PCR Screening for Leishmania ...
Philippe Gil de MENDONCA
doaj   +1 more source

Investigations into the utility of real-time PCR for the detection, quantitation and characterisation of clinically relevant viruses [PDF]

, 2003
The use of PCR as a tool for the diagnostic virology and viral research laboratories has greatly increased in recent years, however the use of conventional PCR and amplicon detection systems can be a complex and relatively slow process that increases the
Mackay, Ian M.
core   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Molecular detection of Mycoplasma gallisepticum by real time PCR [PDF]

, 2014
Mycoplasma gallisepticum (MG) causes chronic respiratory disease leading to huge economic losses to the poultry industry worldwide. Early and efficient detection is therefore crucial in reducing the loss sustained by poultry farmers and poultry industry ...
Ahmad, K., Bejo, Mohd Hair, Farhana Yasmin,, Ideris, Aini, Islam, R., Omar, Abdul Rahman, Tan, C. G., Tan, Sheau Wei   +7 more
core  

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source

Enumeration of Mycobacterium leprae using real-time PCR.

PLoS Neglected Tropical Diseases, 2008
Mycobacterium leprae is not cultivable in axenic media, and direct microscopic enumeration of the bacilli is complex, labor intensive, and suffers from limited sensitivity and specificity. We have developed a real-time PCR assay for quantifying M. leprae
Richard W Truman, P Kyle Andrews, Naoko Y Robbins, Linda B Adams, James L Krahenbuhl, Thomas P Gillis   +5 more
doaj   +1 more source

A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples [PDF]

, 2007
Lutz Lehmann, Klaus‐Peter Hunfeld, Thomas Emrich, Gerd Haberhausen, H. Wissing, Andreas Hoeft, Frank Stüber   +6 more
openalex   +1 more source

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source