Results 161 to 170 of about 20,354,300 (293)

Evaluation of LN34 real-time PCR assay for detection of rabies virus in human and animal specimens. [PDF]

Adv Lab Med
Lodha L, Ashwini MA, Manuel SP, Maladakar A, Chandel S, Bhalke PS, Navya S, Krupa KN, Isloor S, Chandrashekar B, Mahadevan A, Mani RS.   +11 more
europepmc   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Nationwide Survey Using Real-Time PCR in 2024 and 2025 Supports the Absence of Xylella fastidiosa in Korea. [PDF]

Plant Pathol J
Park IW, Park Y, Shin YH, Kim B, Kang B, Kim M, Lee J, Chung M, Cho G, Kim L, Jeon S, Lee S, Lee JY, Hwang J, Seo JY, Yang S, Soe ZZ, Sim HJ, Jung B, Lee SH, Kim JE, Shin J, Lee JH, Jeun YC, Oh CS.   +24 more
europepmc   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

Improved specificity of a probe-based real-time PCR for the detection of Dirofilaria immitis in canine blood. [PDF]

Parasit Vectors
Kelly MA, Sanders TL, Starnes A, Orr E, Luksovsky JL, Sobotyk C, Hakimi H, Weerarathne P, Budke CM, Verocai GG.   +9 more
europepmc   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Robust performance of culture, real-time PCR, and genomic approaches for shigella serotyping in a pediatric surveillance cohort. [PDF]

PLoS Negl Trop Dis
Schiaffino F, Parker CT, Romaina Cachique L, Garcia Bardales PF, Liu J, Peñataro Yori P, Cooper KK, Pascoe B, Pavlinac P, Houpt E, Paredes Olortegui M, Kosek MN.   +11 more
europepmc   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Editorial note: multiplex real-time PCR diagnostic of relapsing fevers in Africa. [PDF]

PLoS Negl Trop Dis
PLOS Neglected Tropical Diseases Editors.
europepmc   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source