Results 261 to 270 of about 1,670,307 (291)
Some of the next articles are maybe not open access.
2008
Although substantial advances have been made in mycobacterial genetics over the past 15 yr, manipulation of mycobacterial genomes and Mycobacterium tuberculosis in particular, continues to be hindered by problems of relatively poor DNA uptake, slow growth rate, and high levels of illegitimate recombination.
Julia C, van Kessel, Graham F, Hatfull
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Although substantial advances have been made in mycobacterial genetics over the past 15 yr, manipulation of mycobacterial genomes and Mycobacterium tuberculosis in particular, continues to be hindered by problems of relatively poor DNA uptake, slow growth rate, and high levels of illegitimate recombination.
Julia C, van Kessel, Graham F, Hatfull
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2013
Homologous recombination is a complex process that has several important roles: repair of broken DNA, repair of disintegrated replication forks and generation of genetic diversity. Two independent recombination pathways, RecBCD and RecF(OR), operate in Escherichia coli. Both pathways are involved in the initial stages of recombination i.e.
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Homologous recombination is a complex process that has several important roles: repair of broken DNA, repair of disintegrated replication forks and generation of genetic diversity. Two independent recombination pathways, RecBCD and RecF(OR), operate in Escherichia coli. Both pathways are involved in the initial stages of recombination i.e.
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Clinical Reviews in Allergy & Immunology, 2001
In the near future, it can be anticipated that recombinant allergens will be used extensively for research purposes, including structural analysis of allergens, determination of T-cell function, and in animal and human experiments on allergen-induced inflammatory reactions.
C, Grégoire, M D, Chapman
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In the near future, it can be anticipated that recombinant allergens will be used extensively for research purposes, including structural analysis of allergens, determination of T-cell function, and in animal and human experiments on allergen-induced inflammatory reactions.
C, Grégoire, M D, Chapman
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Pediatrics In Review, 1991
Cloning and expression of the human gene encoding erythropoietin has resulted in the availability of recombinant erythropoietin for clinical and laboratory investigation. Results of such investigations are clarifying the mechanisms that regulate production of erythropoietin in health and disease.
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Cloning and expression of the human gene encoding erythropoietin has resulted in the availability of recombinant erythropoietin for clinical and laboratory investigation. Results of such investigations are clarifying the mechanisms that regulate production of erythropoietin in health and disease.
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Immunology Today, 1992
The ability of lymphocyte receptor V, D and J gene segments to rearrange generates much of the receptor diversity that is the hallmark of the immune system. Naturally, the mechanisms of immunoglobulin and T-cell receptor gene recombination are of enormous interest.
F W, Alt +5 more
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The ability of lymphocyte receptor V, D and J gene segments to rearrange generates much of the receptor diversity that is the hallmark of the immune system. Naturally, the mechanisms of immunoglobulin and T-cell receptor gene recombination are of enormous interest.
F W, Alt +5 more
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Russian Journal of Bioorganic Chemistry, 2007
An artificial gene encoding oxyntomodulin was obtained using chemical and enzymatic methods and cloned into Escherichia coli. A recombinant plasmid was constructed containing a hybrid oxyntomodulin gene and Ssp dnaB intein from Synechocystis sp. The expression of the resulting hybrid gene in E.
V N, Stepannenko +4 more
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An artificial gene encoding oxyntomodulin was obtained using chemical and enzymatic methods and cloned into Escherichia coli. A recombinant plasmid was constructed containing a hybrid oxyntomodulin gene and Ssp dnaB intein from Synechocystis sp. The expression of the resulting hybrid gene in E.
V N, Stepannenko +4 more
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Autoimmunity Reviews, 2002
Autoimmune diseases frequently manifest themselves in fulminating immune reactions involving T and B cells that lead to the appearance of circulating autoantibodies. Such antibodies are considered to be of diagnostic relevance and may be pathognomonic for their associated autoimmune diseases.
J, Schmitt, W, Papisch
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Autoimmune diseases frequently manifest themselves in fulminating immune reactions involving T and B cells that lead to the appearance of circulating autoantibodies. Such antibodies are considered to be of diagnostic relevance and may be pathognomonic for their associated autoimmune diseases.
J, Schmitt, W, Papisch
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2015
The precise knockout or modification of Mycobacterium tuberculosis genes has been critical for the identification of functions important for the growth and pathogenicity of this important bacterium. Schemes have been previously described, using both non-replicating vectors and transducing particles, for the introduction of gene knockout substrates into
Kenan C, Murphy +2 more
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The precise knockout or modification of Mycobacterium tuberculosis genes has been critical for the identification of functions important for the growth and pathogenicity of this important bacterium. Schemes have been previously described, using both non-replicating vectors and transducing particles, for the introduction of gene knockout substrates into
Kenan C, Murphy +2 more
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Human Antibodies, 1991
Many fundamental advances in our understanding of the structure and function of eukaryotic genes were derived from the study of antibody genes. Examples include mRNA splicing and rearrangement to generate antibody diversity. The capacity to immortalize an individual B cell using cell fusion permitted the generation of monoclonal antibodies.
J W, Larrick, K E, Fry
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Many fundamental advances in our understanding of the structure and function of eukaryotic genes were derived from the study of antibody genes. Examples include mRNA splicing and rearrangement to generate antibody diversity. The capacity to immortalize an individual B cell using cell fusion permitted the generation of monoclonal antibodies.
J W, Larrick, K E, Fry
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Breast Cancer Research and Treatment, 1996
Pseudomonas exotoxin has been genetically modified so that it targets cancer cells. This was accomplished by deleting its cell binding domain and replacing it with Fv fragments of antibodies that react with breast, colon, and other cancers. Several recombinant immunotoxins are now in clinical trials.
I, Pastan +3 more
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Pseudomonas exotoxin has been genetically modified so that it targets cancer cells. This was accomplished by deleting its cell binding domain and replacing it with Fv fragments of antibodies that react with breast, colon, and other cancers. Several recombinant immunotoxins are now in clinical trials.
I, Pastan +3 more
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