Results 81 to 90 of about 611,895 (310)

Recombinant DNA technology and DNA sequencing [PDF]

Essays in Biochemistry, 2019
Abstract DNA present in all our cells acts as a template by which cells are built. The human genome project, reading the code of the DNA within our cells, completed in 2003, is undoubtedly one of the great achievements of modern bioscience.
openaire   +3 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate

Indonesian Journal of Biotechnology, 2015
Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology.
Erma Sulistyaningsih, Sukarti Moeljopawiro, Jarot Subandono, Wayan T. Artama   +3 more
doaj  

New Frontiers in the Production of Functional Recombinant Proteins

Bioengineering
Recombinant protein technology, a pivotal subset of biotechnology, facilitates the generation of specific proteins via recombinant DNA techniques, predominantly within heterologous expression systems [...]
Xin Luo, Yi Ma, Qi Xiang
doaj   +1 more source

Plasmid DNA-based Alphavirus Vaccines

Vaccines, 2019
Alphaviruses have been engineered as vectors for high-level transgene expression. Originally, alphavirus-based vectors were applied as recombinant replication-deficient particles, subjected to expression studies in mammalian and non-mammalian cell lines,
Kenneth Lundstrom
doaj   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Enhanced specific immune responses by CpG DNA in mice immunized with recombinant hepatitis B surface antigen and HB vaccine

Virology Journal, 2011
Background Hepatitis B vaccine adjuvant, alum, is generally used for vaccination although it does not stimulate Th1 immunity and 10% of the population has low or no antibody response.
Wang Xingtai, Hu Zhongyu, He Peng, Zhang Xiancheng, Liang Zhenglun   +4 more
doaj   +1 more source

Commentary: A tribute to Dr. Paul Berg (1926-2023) American biochemist, Nobel Laureate and discoverer of recombinant DNA technology, vaccine and genetic engineering. [PDF]

Front Cell Dev Biol, 2023
Lukiw WJ.
europepmc   +1 more source

The impact of the human DNA topoisomerase II C-terminal domain on activity [PDF]

, 2008
Background Type II DNA topoisomerases (topos) are essential enzymes needed for the resolution of topological problems that occur during DNA metabolic processes.
West, K.L., Katherine L West, Meczes, E.L., Kathryn L Gilroy, Caroline A Austin, Austin Caroline A., Austin, C.A., Gilroy Kathryn L., Meczes Emma L., Gilroy, K.L., Emma L Meczes, West Katherine L.   +11 more
core   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source