Results 201 to 210 of about 255,577 (262)

Fusion tails for the recovery and purification of recombinant proteins

Protein Expression and Purification, 1991
Several fusion tail systems have been developed to promote efficient recovery and purification of recombinant proteins from crude cell extracts or culture media. In these systems, a target protein is genetically engineered to contain a C- or N-terminal polypeptide tail, which provides the biochemical basis for specificity in recovery and purification ...
C F, Ford, I, Suominen, C E, Glatz
openaire   +2 more sources

Antigenicity of a recombinant Ro (SS‐A) fusion protein

Arthritis & Rheumatism, 1990
AbstractThe antigenicity of the 60‐kd human Ro (SS‐A) synthesized in vitro from its complementary DNA as a β‐galactosidase fusion protein (β‐gal—Ro) was evaluated by Western blotting. In this analysis, almost all the anti‐Ro (SS‐A)‐positive sera that bound β‐gal‐Ro also bound affinity‐purified 60‐kd human Ro (SS‐A) (P > 0.005).
J A, James, W D, Dickey, A, Fujisaku, C A, O'Brien, S L, Deutscher, J D, Keene, J B, Harley   +6 more
openaire   +2 more sources

The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins

Molecular Biotechnology, 1999
In the affinity purification of recombinant fusion proteins, the rate-limiting step is usually the efficient proteolytic cleavage and removal of the affinity tail and the protease from the purified recombinant protein. We have developed a rapid, convenient, and efficient method of affinity purification that can overcome this limitation.
openaire   +3 more sources

Recombinant Antibody Fusion Proteins for Cancer Immunotherapy

1996
The last decade has seen the extensive development of monoclonal antibodies (mAb) combined with rapid advances in recombinant DNA technologies. These developments have greatly accelerated and expanded research efforts to generate new approaches for cancer therapy.
R A, Reisfeld, S D, Gillies
openaire   +2 more sources

Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin

Journal of Immunological Methods, 1996
A synthetic DNA encoding human proinsulin was inserted in frame in the bacterial alkaline phosphatase gene. A homogeneous recombinant human proinsulin-alkaline phosphatase conjugate was obtained directly from the periplasm of Escherichia coli transformed with a plasmid carrying the hybrid gene. The recombinant conjugate was stable and could be produced
C, Chanussot, L, Bellanger, C, Ligny-Lemaire, P, Seguin, A, Ménez, J C, Boulain   +5 more
openaire   +2 more sources

Architecting Multicompartmentalized, Giant Vesicles with Recombinant Fusion Proteins

Biomacromolecules
We present a straightforward strategy for constructing giant, multicompartmentalized vesicles using recombinant fusion proteins. Our method leverages the self-assembly of globule-zipper-elastin-like polypeptide fusion protein complexes in aqueous conditions, eliminating the need for organic solvents and chemical conjugation.
Jooyong Shin, Biswajit Saha, Hoyong Chung, Yeongseon Jang   +3 more
openaire   +2 more sources

Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide

Journal of Microbiology, 2022
Dokyun Na, Na Dokyun
exaly  

A recombinant fusion protein clears IgA deposits

Nature Reviews Nephrology, 2022
openaire   +2 more sources

Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins

Journal of Molecular Recognition, 2001
M T Hearn
exaly  

Variability factors in the clinical response to recombinant antibodies and IgG Fc-containing fusion proteins

Expert Opinion on Biological Therapy, 2005
Hervé Watier
exaly