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Recombineering and MAGE [PDF]

open access: possibleNature Reviews Methods Primers, 2021
Recombination-mediated genetic engineering, also known as recombineering, is the genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial genomes. Recombineering and its derivative methods have radically improved genome engineering capabilities, perhaps none more so than multiplex automated genome engineering (MAGE ...
Michaela A. Jones   +16 more
openaire   +3 more sources

Recombinators, recombinases and recombination genes of yeasts [PDF]

open access: possibleCurrent Genetics, 1994
Genetic recombination in the nuclear, organellar, and plasmid genomes of eukaryotic organisms occurs during mitosis and meiosis. Mitotic recombination is an important mechanism for the repair of DNA damaged by ultraviolet light, ionizing radiation, and chemical agents (Friedberg et al. 1991).
Carlo V. Bruschi   +2 more
openaire   +2 more sources
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The Role of Metal Ion Dopants in Quantum-Sized TiO2: Correlation between Photoreactivity and Charge Carrier Recombination Dynamics

, 1994
A systematic study of metal ion doping in quantum (Q)-sized (2-4 nm) TiO_2 colloids is performed by measuring their photoreactivities and the transient charge carrier recombination dynamics.
W. Choi, A. Termin, M. Hoffmann
semanticscholar   +1 more source

Causes and Solutions of Recombination in Perovskite Solar Cells

Advances in Materials, 2018
Organic–inorganic hybrid perovskite materials are receiving increasing attention and becoming star materials on account of their unique and intriguing optical and electrical properties, such as high molar extinction coefficient, wide absorption spectrum,
Jiangzhao Chen, N. Park
semanticscholar   +1 more source

Recombination in yeast and the recombinant DNA technology

Genome, 1989
The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have ...
Martin Kupiec   +9 more
openaire   +2 more sources

Characterizing mutagenic effects of recombination through a sequence-level genetic map

Science, 2019
Human recombination and mutation mapped Genetic recombination is an essential process in generating genetic diversity. Recombination occurs both through the shuffling of maternal and paternal chromosomes and through mutations generated by resolution of ...
B. Halldórsson   +20 more
semanticscholar   +1 more source

Recombination: Meiotic Recombination in Fungi

1997
New combinations of genetic material are generated by the molecular process of genetic recombination. Recombination can affect all types of DNA: nuclear (mitosis and meiosis), organellar and plasmid DNA. Mitotic recombination is rare compared to meiotic recombination and appears to involve a less complex process (Esposito et al.
Heike Röhr, Ursula Kües, Ulf Stahl
openaire   +2 more sources

Mechanism of eukaryotic homologous recombination.

Annual Review of Biochemistry, 2008
Homologous recombination (HR) serves to eliminate deleterious lesions, such as double-stranded breaks and interstrand crosslinks, from chromosomes. HR is also critical for the preservation of replication forks, for telomere maintenance, and chromosome ...
J. San Filippo, P. Sung, H. Klein
semanticscholar   +1 more source

Recombination — Recombination in Higher Plants

1981
Recombination in higher plants is based on events which are coupled to the decisive steps of the sexual cycle, the fusion of the gametes and meiosis; which are introduced by experiments on asexual combinations, e.g., protoplast fusion, organelle transplantation and genetic engi­neering; and which occur in somatic cells, e.g., somatic crossing-over and ...
Reinhard Nehls, Horst Binding
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Recombinant Allergens

Clinical Reviews in Allergy & Immunology, 2001
In the near future, it can be anticipated that recombinant allergens will be used extensively for research purposes, including structural analysis of allergens, determination of T-cell function, and in animal and human experiments on allergen-induced inflammatory reactions.
Christophe Grégoire, Martin D. Chapman
openaire   +3 more sources

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