Results 181 to 190 of about 330,401 (264)

Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach

Biotechnology Progress, EarlyView.
Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya, Rajesh K. Mistry, Susie Sou, Sonja Hess, Bijay Khanal, Zayla Schaeffer, Jie Zhu, Lina Chakrabarti   +7 more
wiley   +1 more source

Cinnamophilin ameliorates testosterone-induced prostatic hyperplasia and fibrosis by regulating 5α-reductase and TGF-β/Smad signaling pathway. [PDF]

Iran J Basic Med Sci
Han D, Chen CY, Huang X, Liu Y, Sun H, Li Y, Liao M, Cai J, Liu J, Li W, Zhang P, Wu Z, Liu CM.   +12 more
europepmc   +1 more source

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐CLOVER establishes enhanced CHO platforms for cell line development

Biotechnology Progress, EarlyView.
This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark Cameron McHargue, Corey Brizzee, Jack Crawford   +7 more
wiley   +1 more source

Bioleaching Microbial Community Metabolism and Composition Driven by Copper Sulphide Mineral Type. [PDF]

Environ Microbiol Rep
Lane KR, Jones SE, Osborne TH, Geller-McGrath D, Nwaobi BC, Chen L, Thomas BC, Hudson-Edwards KA, Banfield JF, Santini JM.   +9 more
europepmc   +1 more source

Construction of a CO2‐Fixing Compartment Using a Shape‐Transforming DNA Scaffold

Chemistry – A European Journal, EarlyView.
A shape‐transforming DNA scaffold encapsulates RuBisCO within a controllable, self‐contacting compartment that mimics the carboxysome. The scaffold switches between open and closed states, enabling direct comparison of identical enzyme populations in different microenvironments.
Hui Yang, Peng Lin, Surachada Chuaychob, Huyen Dinh, Mei Nakabayashi, Eiji Nakata, Takashi Morii   +6 more
wiley   +1 more source

Molecular evolution and diversity of isomerase-reductase clusters involved in the bacterial metabolism of glycosaminoglycans. [PDF]

mSphere
Nishimura Y, Okumura K, Oiki S, Ogura K, Hashimoto W.   +4 more
europepmc   +1 more source

Chemo‐Enzymatic Route for Covalent Cellulose Fiber Dyeing

Chemistry – A European Journal, EarlyView.
This work presents a proof of concept for a chemo‐enzymatic approach to the covalent dyeing of cotton fibers. Laccase/TEMPO oxidation introduces aldehyde groups, enabling subsequent reductive amination with amino dyes. Excellent color homogeneity and wash fastness were demonstrated using acid red 33, highlighting the robustness of the approach and its ...
Awilda Maccow, Etienne Severac, Sandrine Morel, Claire Moulis, Guillaume Boissonnat, Magali Remaud‐Simeon, David Guieysse   +6 more
wiley   +1 more source

Glutamine Substitution at Lysine⁶⁰⁹ in the Autoinhibitory Region Modulates Catalysis, Uncoupling, and Conformational Dynamics of Endothelial Nitric Oxide Synthase. [PDF]

ACS Omega
Masood M, Khan S, Yameen D, Anwar A, Khan F, Iqbal S, Chaturvedi R, Shamsi A, Haque MM.   +8 more
europepmc   +1 more source

Asymmetric Reduction of Unactivated Alkenes

Chemistry – A European Journal, EarlyView.
Unactivated alkenes rank among the most inert functional groups in synthesis and their selective reduction remains challenging. This Review charts the evolution from classical metal‐catalyzed hydrogenation to radical hydrogen atom transfer (HAT) and emerging biocatalytic concepts, highlighting how complementary mechanistic strategies, including the ...
Nico D. Fessner, Sebastian Roth, Richard Niese, Michael Müller   +3 more
wiley   +1 more source