Results 111 to 120 of about 516,580 (306)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Points of Reference in Validation Process [PDF]

, 2008
In the validation of toxicological test methods the test results are usually assessed by comparison/correlation with results derived from currently used tests in accordance with existing regulations, policies, guidelines and guidances, and which are, in ...
NIKOLAIDIS Efstathios, EDLER Lutz, GARDNER Ian, HARTUNG Thomas, KLEIN Christoph, LIEBSCH Manfred, HOFFMANN Sebastian, STAMMATI Annalaura, SAUERLAND Stefan, GRIBALDO Laura, SCHECHTMAN Leonard   +10 more
core  

Contribution of PPP with Ambiguity Resolution to the Maintenance of Terrestrial Reference Frame

Remote Sensing
Precise Point Positioning (PPP) provides static positioning at the millimeter level and kinematic positioning ranging from millimeters to decimeters globally.
Ruyuan Wang, Junping Chen, Yize Zhang, Weijie Tan, Xinhao Liao   +4 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Social effects on reference frame selection [PDF]

, 2018
The presence of another person in a spatial scene has been shown to induce spontaneous perspective taking. This investigation presents two experiments exploring whether the presence of another person affects reference frame selection when representing ...
Cherep, Lucia, Costabile, Kristi, Kelly, Jonathan   +2 more
core   +1 more source

Best Practice for Integrating InSAR and GNSS Observations in High-Density GNSS Networks

IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing
Interferometric synthetic aperture radars (InSAR) is widely used to monitor ground deformation, but its results are relative to a local satellite-based reference system.
Eugenia Giorgini, Milan Lazecky, Andrew Hooper, Stefano Gandolfi   +3 more
doaj   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Motor biases reflect a misalignment between visual and proprioceptive reference frames

eLife
Goal-directed movements can fail due to errors in our perceptual and motor systems. While these errors may arise from random noise within these sources, they also reflect systematic motor biases that vary with the location of the target.
Tianhe Wang, J Ryan Morehead, Amber Jiang, Richard B Ivry, Jonathan S Tsay   +4 more
doaj   +1 more source