Results 171 to 180 of about 11,409,850 (299)

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Optimization of tablet processing as a reference material for microplastic detection methods. [PDF]

Anal Bioanal Chem
Putzu M, Wiesner Y, Weimann C, Hodoroaba VD, Muniategui Lorenzo S, Fernández-Gonzáles V, Booth AM, Igartua A, Benismail N, Coïc L, Chivas-Joly C, Fenoglio I, Rossi AM, Giovannozzi AM, Altmann K.   +14 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Development and validation of a national reference material system for quality control of chikungunya virus nucleic acid detection assays. [PDF]

J Clin Microbiol
Ma T, Zhao L, Luo C, Wang Y, Tian Y, Xu S.   +5 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A New Matrix Certified Reference Material for Measurement of Chlormequat Chloride and 2,4-Dichlorophenoxyacetic Acid Residues in Cucumber. [PDF]

Foods
Li L, Xu Q, Shi H, Yang M, Yan J, Zhou J, Li F, Li L, Wang M.   +8 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

A reference material for X-ray diffraction line profile analysis. [PDF]

J Appl Crystallogr
Scardi P, D'Incau M, Malagutti MA, Terban MW, Hinrichsen B, Fitch AN.   +5 more
europepmc   +1 more source

Development of a Pure Certified Reference Material of D-Mannitol. [PDF]

Molecules, 2023
Chen W, Zhang Y, Chen H, Jin W, Chen X, Huang X, Xie Y, Fang H, Hong Z.   +8 more
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source