Results 81 to 90 of about 1,698,809 (169)

Pseudovirus as an Emerging Reference Material in Molecular Diagnostics: Advancement and Perspective. [PDF]

Curr Issues Mol Biol
Zheng L, Xu S.
europepmc   +1 more source

Development of Human Rhinovirus RNA Reference Material Using Digital PCR. [PDF]

Genes (Basel), 2023
Ju DU, Park D, Kim IH, Kim S, Yoo HM.
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Development of a Mixed Solution Certified Reference Material of Vanillin Spices. [PDF]

ACS Omega
Ma J, Luo S, Li X, Zhao X, Li X, Zhang Q, Yin X.   +6 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Correction to: Development of Psilocybe Mushroom Species Reference Material-Cultivation Parameters and Chemical Profiles. [PDF]

J AOAC Int
europepmc   +1 more source

Hydroculture Cultivation of Strawberries as Potential Reference Material for Microcystin Analysis: Approaches and Pitfalls. [PDF]

Toxins (Basel)
Van Hassel WHR, Guillaume B, Masquelier J.   +2 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Digital PCR-Based characterization of a Zhonghuang 6106 soybean genomic DNA reference material for its food and feed detection. [PDF]

Sci Rep
Wang C, Li R, Liu N, Qi X, Xu C, Yao J, Zhang C, Wang Y, Lan Q, Wang Y, Zhao X.   +10 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source