Results 211 to 220 of about 123,764 (312)

Rapid spread of the emerging cosmopolitan genotype of dengue virus serotype 2, and expansion of dengue virus serotype 1 genotype V in Peru. [PDF]

Rev Peru Med Exp Salud Publica
Bailon H, Jimenez V, Galarza M, Medrano P, Mestanza O, Figueroa D, Lizarraga W, Silva I, Sevilla L, Hurtado V, Izarra V, Padilla C, Barcena L, Caceres O, Merino S, Marcelo A, Ruiz N, Hapuarachchii HC, Sánchez CC, García MP.   +19 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Mercados de trabajo en la agricultura mediterránea [PDF]

, 2012
Saldaña Ramírez, Adriana, Reigada Olaizola, Alicia   +1 more
core  

[Implementation sciences: Conceptual frameworks and an illustrative example of PRONAM]. [PDF]

Rev Med Inst Mex Seguro Soc
McCutchan T, López-Sánchez M, Rivas-Ruiz R.   +2 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Neuroplasticity in Post-Traumatic Stress Disorder. [PDF]

Rev Neurol
López-López B, Crespo I.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

[Cardiovascular risk in adults of indigenous descent in Argentina: an analysis of the Second National Nutrition and Health Survey] [PDF]

Rev Fac Cien Med Univ Nac Cordoba
Awruch C, Solarz F, Pérez Bridoux F, Zamora MF, Mattig ME, Cruz CV, Tentoni N, Politi MT.   +7 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source