Results 231 to 240 of about 1,489,667 (310)

PETRA: Prime editing of transcribed regulatory elements to assay expression

Reyes MA, Herger M, Cubitt L, Findlay GM.   +3 more
europepmc   +1 more source

Unveiling Epigenetic Regulatory Elements Associated with Breast Cancer Development. [PDF]

Int J Mol Sci
Jardanowska-Kotuniak M, Dramiński M, Wlasnowolski M, Łapiński M, Sengupta K, Agarwal A, Filip A, Ghosh N, Pancaldi V, Grynberg M, Saha I, Plewczynski D, Dąbrowski MJ.   +12 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Compensatory action of different types of cis-regulatory elements buffers the striped expression of Drosophila pair-rule genes. [PDF]

Development
Fischer MD, Au K, Lê M, Graham P, Pick L.   +4 more
europepmc   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Structural Characterization of <i>DDX23</i> 5' UTR Regulatory Elements and Their Targeting by LNA-Modified Antisense Oligonucleotides. [PDF]

Int J Mol Sci
Kamzeeva P, Shepelev N, Zabbarova V, Brylev V, Chistov A, Ryazantsev D, Kot E, Novopashina D, Rubtsova M, Aralov A.   +9 more
europepmc   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic loci. [PDF]

Cell Genom
Zhao K, Zhou Y, Wang X, Wang Z, Xu X, Chen Y, Zhao L, Wu C, Wang J, Yao H, Cheng X, Wang W, Chu X, Wang W, Yi X, Chen Y, Li M, Lu W, Chen K, Sham PC, Huang D, Zhang J, Li MJ.   +22 more
europepmc   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source