Results 161 to 170 of about 1,830,137 (233)

The Microbiology and Geochemistry of the Shallow-Water Hydrothermal Vents of the Gulf of Naples, Italy. [PDF]

Environ Microbiol
Barosa B, Celentano C, Migliaccio F, Diana SC, Silva ACP, Selci M, Tonietti L, Bastoni D, Cascone M, Bastianoni A, Correggia M, di Iorio L, Price R, Caliro S, Milazzo M, Aiuppa A, Vetriani C, Cordone A, Giovannelli D.   +18 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

A Reaction-Diffusion Model for Capturing Mass Loss and Microstructure Evolution in Enzymatic Degradation of Poly(<i>ε</i>-Caprolactone) Films. [PDF]

Polymers (Basel)
Nansak N, Creedon L, O'Mahoney D, Ghosh R, McAfee M.   +4 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Liquid crystal-like self-organization of glioblastoma is associated with consistent migration for high cell densities. [PDF]

Sci Rep
Hohmann U, Ghadban C, Prell J, Strauss C, Dehghani F, Hohmann T.   +5 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Repeatability of Knee Extension Muscle Endurance Between 20% and 40% of One Repetition Maximum. [PDF]

Muscles
Hillen SJ, Fliss MD, Mitchell CJ.
europepmc   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

Listeners near-optimally integrate acoustic and semantic cues in spoken word recognition: Evidence from experiments manipulating cue order and reliability. [PDF]

Atten Percept Psychophys
Westwig A, Kim Y, Bushong W.
europepmc   +1 more source