Results 31 to 40 of about 671,273 (211)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Land, 2022 Through recognition and mastery of the regional differences and influencing factors of China’s rural housing rental rates, we can better understand changes in the functional attributes of homesteads and deepen the reform of “separating rural land ...
Li Huang, Minjie Zheng, Rongyu Wang
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Increase Government Support for Build-to-Rent: Should We Expect Affordable Housing?
Buildings, 2023 Build-to-rent (BTR) is an important pathway for rental housing supply across the cities of Australia. The discourse has become more prevalent as stakeholders seek government support to expand the sector to provide, amongst other reform measures ...
Samuel Swanzy-Impraim +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
New Directions for Adult and Continuing Education, EarlyView.ABSTRACT Advancing artificial intelligence (AI) has transformed learning and work, yet higher education and professional development programs have not systematically equipped learners for AI‐prevalent environments. This lack of preparation creates uncertainty regarding control, responsibility, trust, and accountability.
Moon‐Heum Cho, Jerusalem Merkebu
wiley +1 more source
Housing Need and Housing Pressures in St Andrews [PDF]
, 2014 Funding: The St Andrews Preservation Trust, St Andrews Common Good Fund, University of St Andrews, anonymous donationsIn 2012 a group of local organisations resolved to promote a serious study of the housing situation in the town. To carry out this task,
Grant, Ian +4 more
core +2 more sources
Measurement: SensorsSmart cities leverage technology, data, and digital infrastructure to enhance residents' quality of life, fostering sustainability and operational efficiency. To this end, the price data of rental housing in Hefei in 2022 were hereby collected to build a
Wen Zhao, Jie Zhong, Jiale Lv
doaj +1 more source
β‐Catenin/c‐Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations
Advanced Biology, Volume 9, Issue 3, March 2025.Ammonia, detoxified by the liver into urea and glutamine, impacts autophagy differently at varying levels. Low ammonia activates autophagy via c‐Myc and β‐catenin, while high levels suppress it. Using Huh7 cells and Spf‐ash mice, c‐Myc's role in cytoprotective autophagy is revealed, offering insights into hyperammonemia and potential therapeutic ...
S. Sergio +11 more
wiley +1 more source