Nanostructured Hydrogels: A Method to Prevent Biofilms on Implantable Medical Devices. [PDF]
Jayasinghe HG +2 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
A Simulation-Based Approach to Procedural Education in Resource-Limited Settings: Designing an Abdominal Trainer for Paracentesis and Lumbar Puncture. [PDF]
Hayden A +4 more
europepmc +1 more source
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
Fabricating Microstructures on Freeform Surfaces via Flexible Hydrogel Micromolds. [PDF]
Zhu P +5 more
europepmc +1 more source
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
Photopolymer Flexographic Printing Plate Mold for PDMS Microfluidic Manufacture. [PDF]
Peñaherrera-Pazmiño AB +3 more
europepmc +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Dextran-modified dissolving microneedle patches for effective amelioration of psoriasis through transdermal delivery of hesperidin. [PDF]
Etemadi N +4 more
europepmc +1 more source

