Results 161 to 170 of about 876,186 (303)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Does genetic rescue disrupt local adaptation? An experimental test using thermally adapted <i>Tribolium castaneum</i> lines. [PDF]

Proc Biol Sci
West G, Pointer MD, Nash WJ, Lewis R, Richardson DS.   +4 more
europepmc   +1 more source

Pioglitazone plus (−)‐epigallocatechin gallate: a novel approach to enhance osteogenic performance in aged bone marrow mesenchymal stem cells

FEBS Open Bio, EarlyView.
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen, Jia‐Ci Jhang, Chun‐Yi Peng, Quốc Cường Nguyễn, Feng‐Huei Lin, Hung‐Ming Wu, Shankung Lin   +6 more
wiley   +1 more source

Effectiveness and safety of techniques for cervical spine immobilization in mountain rescue. [PDF]

Scand J Trauma Resusc Emerg Med
Kraus RF, Knipfer ML, Jacob M, Kieninger B, Alikhani J, Ghamsary PH, Reinker L, Adler I, Dendorfer S, Kieninger M.   +9 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Comparison of oral versus rectal NSAIDs for perioperative analgesia in pediatric circumcision using a disposable circumcision suture device: a randomized controlled trial. [PDF]

BMC Anesthesiol
Zhou J, Ouyang Y, Hu S, Xiong Y, Liu M.
europepmc   +1 more source

Rescue works led in objects with bounded space

Bezpieczeństwo i Technika Pożarnicza, 2009
Статья описывает специфику спасательных действий проводимых в колодцах, силосных башнях и разного рода каналах и объектах, характеризующихся очень ограниченным пространством. Представляет угрозы, характерные для этих объектов, а также оборудование для проведения эффективных действий и техники спасения.The article describes the specific of rescue works ...
openaire   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Retention of survival swimming skills among SwimSafe graduates in rural communities of Bangladesh: Results from a cross-sectional study. [PDF]

Inj Prev
Rahman A, Ashraf L, Rahman AF, Bhuiyan MA, Shafkat Hossain M, Alam Z, Talab A, Li Q, Bachani A.   +8 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source