Results 191 to 200 of about 56,063,487 (256)

Variability in research culture across busy catheterisation labs in the Asia-Pacific region. [PDF]

AsiaIntervention
Narang M, Saxena A, Kaur R, Gopa HR, West NEJ.   +4 more
europepmc   +1 more source

Australian Centre for Christianity and Culture

Report on the Australian Centre for Christianity and ...
Australian Centre for Christianity and Culture
core  

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Research culture in biomedicine: what we learned, and what we would like to do about it. [PDF]

Commun Biol
McCray AT, Van Vactor D, Gould J, Li X, Patrnogić J, Shamu C, Walsh MC.   +6 more
europepmc   +1 more source

Next generation diabetes scientists shape global research culture: A reflective proposal from postdoctoral researchers in diabetes research. [PDF]

Acta Physiol (Oxf), 2020
Attendees of the Danish Diabetes Academy Winter School 2019.
europepmc   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Protocol for a theory-based, mixed methods evaluation of Cynnau|Ignite: an active learning programme to foster positive research culture through leadership development at Cardiff University. [PDF]

PLoS One
Hennah C, Rizwan M, Edwards N, Beckett H, Convery M, Dawson H, Morris J, Rigby M, Vilares J, Wahl-Jorgensen K, Williams R, Gameiro S.   +11 more
europepmc   +1 more source

The role of Allied Health Professions and Nursing Research Internships in developing a research culture: a mixed-methods exploration of stakeholder perspectives. [PDF]

Health Res Policy Syst, 2020
Nightingale J, Fowler-Davis S, Grafton K, Kelly S, Langham C, Lewis R, Bianco B, Harrop D.   +7 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source