Results 21 to 30 of about 72,264 (215)

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Integrated model "reservoir-well" based on D-CRMP

open access: yesИзвестия высших учебных заведений: Нефть и газ
The active use of integrated modeling in reservoir engineering of oil fields has led to research to overcome the problems of its practical application.
V. I. Lebedev   +3 more
doaj   +1 more source

Dissipative Synthesis of Mechanical Fock-Like States

open access: yesProceedings, 2019
The observation of genuine quantum features of nano-mechanical motion is a key goal for both fundamental and applied quantum science. To this end, a promising approach is the stabilization of nonclassical features in the presence of dissipation, by means
Matteo Brunelli, Oussama Houhou
doaj   +1 more source

Stationary Gaussian entanglement between levitated nanoparticles

open access: yesNew Journal of Physics, 2020
Coherent scattering of photons is a novel mechanism of optomechanical coupling for optically levitated nanoparticles promising strong, versatile interactions with light and between nanoparticles.
Anil Kumar Chauhan   +2 more
doaj   +1 more source

Economic and fault stability analysis of geothermal field development in direct-use hydrothermal reservoirs

open access: yesGeothermal Energy, 2021
The installed capacity of geothermal systems for direct use of heat is increasing worldwide. As their number and density is increasing, the their interaction with subsurface faults becomes more important as they could lead to safety risks from induced ...
Caroline Zaal   +2 more
doaj   +1 more source

Metastasis on pause: How dormant tumor cells stay hidden within the tumor microenvironment and evade immune surveillance

open access: yesMolecular Oncology, EarlyView.
Dormant cancer cells can hide in distant organs for years, evading treatment and the immune system. This review highlights how signals from the surrounding tissue and immune environment keep these cells inactive or trigger their reawakening. Understanding these mechanisms may help develop therapies to eliminate or control dormant cells and prevent ...
Kanishka Tiwary   +1 more
wiley   +1 more source

Experimental investigations of oil flow under pressure below bubble point pressure

open access: yesGeoresursy
The article considers the features of live oil flow investigation under pressure below bubble point pressure and the methodology for performing special core analysis.
I. I. Ibragimov   +3 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Estimating uncertainties in 3-D models of complex fold-and-thrust belts: A case study of the Eastern Alps triangle zone

open access: yesApplied Computing and Geosciences, 2023
Geological modeling commonly results in a single prescribed geometric representation of the subsurface with no consideration of uncertainties. Accounting for uncertainties is of particular importance in the triangle zone at the leading edge of ...
Sofia Brisson   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

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