Results 51 to 60 of about 21,625,103 (249)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Residence time distribution in continuous virus filtration

Biotechnology and Bioengineering
Regulatory authorities recommend using residence time distribution (RTD) to address material traceability in continuous manufacturing. Continuous virus filtration is an essential but poorly understood step in biologics manufacturing.
Yu‐Cheng Chen, Gabriele Recanati, Fernando De Mathia, Dong‐Qiang Lin, Alois Jungbauer   +4 more
openaire   +2 more sources

A one dimensional model for the prediction of extraction yields in a two phases modified twin-screw extruder [PDF]

, 2002
Solid/liquid extraction is performed on raw plant substrate with a modified twin-screw extruder (TSE) used as a thermo-mecanochemical reactor. Visual observations and experimental residence time distributions (RTD) are used to develop a solid transport ...
Prat, Laurent E., Guiraud, Pascal, Prat, Laurent, Gourdon, Christophe, Rigal, Luc, L. Prat, P. Guiraud, L. Rigal, C. Gourdon   +8 more
core   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

Understanding the Residence Time Distribution in a Transient Inline Spiking System: Modeling, Experiments, and Simulations. [PDF]

Membranes (Basel), 2023
Hwang M, Wang J, Jung SY.
europepmc   +1 more source

Universality of residence-time distributions in non-adiabatic stochastic resonance [PDF]

, 2004
International audienceWe present a mathematically rigorous expression for the residence-time distribution of a periodically forced Brownian particle in a bistable potential.
Berglund, Nils, B. Gentz, Gentz, Barbara, N. Berglund   +3 more
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Residence Time Distribution of Multiple Particles in Four Configurations of Holding Tubes

, 2004
Residence time distributions of multiple particles (as affected by process and system parameters) were investigated during non-Newtonian tube flow. Process parameters included flow rate, particle type, carrier fluid viscosity, and particle concentration.
Tanyel, Zeynep
core