Results 41 to 50 of about 29,160 (257)

The Identification of Sporopollenin in Sections of Resin-Embedded Tissues by Controlled Acetolysis

Stain Technology, 1973
Using a modification of the acetolysis technique it is possible to isolate sporopollenin-rich structures in sections of epoxy-resin embedded plant material. An advantage of the technique, which consists of heating a 9:1 acetic anhydride/H2SO4 mixture to boiling for about 5 min under a cover glass applied to a slide bearing the mounted sections, is that
Dickinson, H, Bell, P
openaire   +3 more sources

Fabrication of Microstructures Embedding Controllable Particles inside Dielectrophoretic Microfluidic Devices

International Journal of Advanced Robotic Systems, 2013
This paper presents a method of particle manipulation by dielectrophoresis (DEP) and immobilization using photo-crosslinkable resin inside microfluidic devices.
Tao Yue, Masahiro Nakajima, Hirotaka Tajima, Toshio Fukuda   +3 more
doaj   +1 more source

Influence of Test Temperature and Test Frequency on Fatigue Life of Aluminum Alloy EN AW‐2618A

Advanced Engineering Materials, EarlyView.
The influence of test temperature and test frequency on the fatigue life of EN AW‐2618A is investigated. High‐cycle fatigue tests are performed at different test temperatures and frequencies on the 1000 h/230°C overaged state. Both test parameters reduce fatigue life due to time‐dependent damage mechanisms.
Ying Han, Julian M. Rosalie, Jan Radners, Mauro Madia, Philipp von Hartrott, Birgit Skrotzki   +5 more
wiley   +1 more source

Rapid in-EPON CLEM: Combining fast and efficient labeling of self-labeling enzyme tags with EM-resistant Janelia Fluor dyes and StayGold

Heliyon
Correlative light and electron microscopy (CLEM) combines light microscopy (LM) of fluorescent samples to ultrastructural analyses by electron microscopy (EM).
Rico Franzkoch, Sabrina Wilkening, Viktoria Liss, Michael Holtmannspötter, Rainer Kurre, Olympia E. Psathaki, Michael Hensel   +6 more
doaj   +1 more source

MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

eLife, 2019
The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable.
Thomas Templier
doaj   +1 more source

Microstructure Reconstruction in Battery Electrodes Using Machine Learning Based on Low‐Voltage Focused Ion Beam–Scanning Electron Microscopy Tomography Images

Advanced Engineering Materials, EarlyView.
Low‐voltage FIB‐SEM tomography combined with a image preprocessing pipeline improves phase contrast and enables reliable machine‐learning segmentation of conductive networks in lithium‐ion battery electrodes. Structural descriptors are extracted from segmented images, done semimanually and automated, and compared.
Lisa Beran, Vincent Nebel, Dominik Perius, Kshitij Kumar, Yannis Heim, Laura Bies, Tobias Kraus   +6 more
wiley   +1 more source

LR White is preferable to Unicryl for immunogold detection of fixationsensitive nuclear antigens

European Journal of Histochemistry, 2010
The purpose of this study was to compare two electron microscopy embedding media – LR White and Unicryl – with regard to cell morphologyical and immunohistochemical preservation properties for the study of fixation-sensitive nuclear antigens.
V. V. Philimonenko, J. Janácek, P. Hozák   +2 more
doaj   +1 more source

Inverse Identification of Energy‐Dependent Laser Absorptivity in NiTi Laser Powder‐Bed Fusion via Calibrated Melt Pool Simulation

Advanced Engineering Materials, EarlyView.
A combined experimental–computational framework identifies energy‐dependent laser absorptivity for NiTi in laser powder‐bed fusion, applicable to conduction and transition modes. Single‐track experiments and thermofluid smoothed particle hydrodynamics simulations are coupled through inverse analysis of melt pool geometry.
Mohamadreza Afrasiabi, Hadi Moutablaleh, Ignatius A. Setiawan, Mehrshad Mehrpouya   +3 more
wiley   +1 more source

Enzyme histochemistry on freeze-dried, resin-embedded tissue. [PDF]

Journal of Histochemistry & Cytochemistry, 1989
We have developed a method for histochemical demonstration of a wide range of enzymes in freeze-dried, resin-embedded tissue. Freeze-dried tissue specimens were embedded without fixation at low temperature (4 degrees C or -20 degrees C) in glycol methacrylate resin or LR Gold resin.
G I, Murray, M D, Burke, S W, Ewen
openaire   +2 more sources

A Practical Noise2Noise Denoising Pipeline for High‐Throughput Raman Spectroscopy

Advanced Engineering Materials, EarlyView.
A lightweight and reproducible denoising pipeline for high‐throughput Raman spectroscopy is introduced, based on a 1D convolutional autoencoder trained with a Noise2Noise strategy. Using only repeated short‐exposure acquisitions, the method suppresses stochastic noise without reference spectra, enabling reliable spectral reconstruction while preserving
David Martin‐Calle, Cesar Alvarez‐Llamas, Vincent Motto‐Ros, Christophe Dujardin, Jérémie Margueritat, David Rodney   +5 more
wiley   +1 more source