Results 201 to 210 of about 612,357 (298)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

UbaLAI is a monomeric Type IIE restriction enzyme. [PDF]

Nucleic Acids Res, 2017
Sasnauskas G, Tamulaitiene G, Tamulaitis G, Calyševa J, Laime M, Rimšeliene R, Lubys A, Siksnys V.   +7 more
europepmc   +1 more source

Correction to: Dead Cas9-sgRNA Complex Shelters Vulnerable DNA Restriction Enzyme Sites from Cleavage for Cloning Applications, by Saifaldeen M et al. The CRISPR Journal, 2021;4(2):275-289; DOI: 10.1089/crispr.2020.0134. [PDF]

CRISPR J, 2021
europepmc   +1 more source

Changes in Restriction Enzyme Pattern of the Equine Herpes Virus Type 1 (EHV-1) Strain RacH DNA During Attenuation [PDF]

, 1987
Allen, Allen, Borgen, Chowdhury, Davison, Klingeborn, Lomniczi, Maniatis, Mayr, Studdert, Studdert, Whalley   +11 more
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Expanding the scope of methylation-sensitive restriction enzyme (MSRE) PCR for forensic identification of body fluids through the novel use of methylation-dependent restriction enzymes (MDRE) and the combination of autosomal and Y-chromosomal markers. [PDF]

Int J Legal Med
Rothe J, Becker JM, Charchinezhadamouei M, Mähr S, Lembeck F, Dannemann N, Nagy M.   +6 more
europepmc   +1 more source

MTHFR C677T polymorphism analysis: A simple, effective restriction enzyme-based method improving previous protocols. [PDF]

Mol Genet Genomic Med, 2019
Antonaros F, Olivucci G, Cicchini E, Ramacieri G, Pelleri MC, Vitale L, Strippoli P, Locatelli C, Cocchi G, Piovesan A, Caracausi M.   +10 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

One-tube restriction enzyme digest and fluorescent labeling for restriction endonuclease fingerprinting single-strand conformational polymorphism

BioTechniques, 2004
Ove Bruland, Per Morten Knappskog
doaj   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source