Results 91 to 100 of about 798,636 (312)

Meiotic maps of sockeye salmon derived from massively parallel DNA sequencing

BMC Genomics, 2012
Background Meiotic maps are a key tool for comparative genomics and association mapping studies. Next-generation sequencing and genotyping by sequencing are speeding the processes of SNP discovery and the development of new genetic tools, including ...
Everett Meredith V, Miller Michael R, Seeb James E   +2 more
doaj   +1 more source

Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation

FEBS Letters, EarlyView.
The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt, Marcus S. Münchhoff, Jacqueline Krohn, Philip M. Palarz, Manuel H. Taft, Johannes N. Greve, Nataliya Di Donato, Falk F. R. Buettner, Dietmar J. Manstein   +8 more
wiley   +1 more source

Assessment of groundwater quality for irrigation purpose using irrigation water quality index (IWQI)

Journal of Environmental Engineering and Landscape Management
Groundwater represents an important natural resource for sustaining life. This study was conducted to evaluate groundwater in the Doukkala region in Morocco, using the Irrigation Water Quality Index (IWQI) and uses Inverse Distance Weighting (IDW) in ...
Hefdhallah S. Al-Aizari, Fatima Aslaou, Osan Mohsen, Ali R. Al-Aizari, Abdel-Basit Al-Odayni, Naaser A. Y. Abduh, Abdul-Jaleel M. Al-Aizari, Eman Abo Taleb   +7 more
doaj   +1 more source

Molecular Mapping of Restriction-Site Associated DNA Markers In Allotetraploid Upland Cotton.

PLoS ONE, 2015
Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers is difficult. The recent emergence of genome complexity reduction technologies based on the next-generation
Yangkun Wang, Zhiyuan Ning, Yan Hu, Jiedan Chen, Rui Zhao, Hong Chen, Nijiang Ai, Wangzhen Guo, Tianzhen Zhang   +8 more
doaj   +1 more source

Type II Restriction of Bacteriophage DNA With 5hmdU-Derived Base Modifications

Frontiers in Microbiology, 2019
To counteract bacterial defense systems, bacteriophages (phages) make extensive base modifications (substitutions) to block endonuclease restriction.
Kiersten Flodman, Rebecca Tsai, Michael Y. Xu, Ivan R. Corrêa, Alyssa Copelas, Yan-Jiun Lee, Ming-Qun Xu, Peter Weigele, Shuang-yong Xu   +8 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Locating Restricted Facilities on Binary Maps

, 2008
In this paper we consider several facility location problems with applications to cost and social welfare optimization, when the area map is encoded as a binary (0,1) mxn matrix. We present algorithmic solutions for all the problems. Some cases are too particular to be used in practical situations, but they are at least a starting point for more ...
Mugurel Ionut Andreica, Cristina Teodora Andreica, Madalina Ecaterina Andreica   +2 more
openaire   +4 more sources

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Using Linkage Maps as a Tool To Determine Patterns of Chromosome Synteny in the Genus Salvelinus

G3: Genes, Genomes, Genetics, 2017
Next generation sequencing techniques have revolutionized the collection of genome and transcriptome data from nonmodel organisms. This manuscript details the application of restriction site-associated DNA sequencing (RADseq) to generate a marker-dense ...
Matthew C. Hale, Garrett J. McKinney, Courtney L. Bell, Krista M. Nichols   +3 more
doaj   +1 more source