Results 291 to 300 of about 666,471 (318)

Reverse Genetics and Cystic Fibrosis

American Journal of Respiratory Cell and Molecular Biology, 1990
The protein responsible for cystic fibrosis has been identified using an approach called "reverse" genetics. This approach relies on the chromosomal map position to direct the search for a disease gene, several novel cloning strategies to isolate the gene, and the gene's sequence to define the abnormal protein.
M C, Iannuzzi, F S, Collins
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Reverse Genetics of Dengue Virus

2023
Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering its epidemiological importance, efforts have been directed towards understanding various aspects of DENV biology, which have been facilitated by the development of different molecular strategies for ...
José Valter Joaquim, Silva Júnior, Andréa Nazaré Monteiro Rangel, da Silva, Jefferson José, da Silva Santos, Laura Helena Vega Gonzales, Gil   +3 more
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Reverse Genetics of Influenza Viruses

2012
The ability to modify influenza viruses at will has revolutionized influenza research. Reverse genetics has been used to generate mutant or reassortant influenza viruses to assess their replication, virulence, pathogenicity, host range, and transmissibility.
Gabriele, Neumann, Makoto, Ozawa, Yoshihiro, Kawaoka   +2 more
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Genetics and Reverse Genetics of Nidoviruses

2014
The genomes of nidoviruses are infectious, and virus replication is initiated as the genome is delivered to the cytoplasm and the replicase is translated by the host cell ribosomes. Nidovirus reverse-genetics systems are needed to better understand aspects of their complex replication strategy, pathogenesis, and mechanisms of host range expansion, and ...
Damon J. Deming, Ralph S. Baric
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Reverse Genetics of Zika Virus

2017
We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A
Chao, Shan, Xuping, Xie, Pei-Yong, Shi
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Prions: Pathogenesis and Reverse Genetics

Annals of the New York Academy of Sciences, 2000
Abstract: Spongiform encephalopathies are a group of infectious neurodegenerative diseases. The infectious agent that causes transmissible spongiform encephalopathies was termed prion by Stanley Prusiner. The prion hypothesis states that the partially protease‐resistant and detergent‐insoluble prion protein (PrPsc) is identical with the infectious ...
Aguzzi, A, Klein, M A, Montrasio, F, Pekarik, V, Brandner, S, Furukawa, H, Käser, P, Röckl, C, Glatzel, M   +8 more
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Reverse genetics

2020
This chapter focuses on reverse genetics in which the start point is the DNA sequence of a cloned gene, and the mutant phenotype—and hence biological function of the gene—is inferred from that sequence. The chapter starts with an exploration of the reverse genetic analysis carried out on budding yeast and mice.
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Reverse Genetic Approaches in Zebrafish

Journal of Genetics and Genomics, 2012
Zebrafish (Danio rerio) is a well-established vertebrate animal model. A comprehensive collection of reverse genetics tools has been developed for studying gene function in this useful organism. Morpholino is the most widely used reagent to knock down target gene expression post-transcriptionally.
Peng, Huang, Zuoyan, Zhu, Shuo, Lin, Bo, Zhang   +3 more
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Reverse Genetics Systems for Filoviruses

2017
Filoviruses are among the most pathogenic viruses known to man. Reverse genetics systems, in particular full-length clone systems, allow the generation of recombinant filoviruses, which can be used to study virus biology, but also for applied uses such as screening for countermeasures.
Thomas, Hoenen, Heinz, Feldmann
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Lassa Virus Reverse Genetics

2017
The Old World (OW) arenavirus Lassa (LASV ) is estimated to infect several hundred thousand people yearly in West Africa, resulting in high numbers of Lassa fever (LF), a viral hemorrhagic fever (HF) disease associated with high morbidity and mortality.
Luis, Martínez-Sobrido, Slobodan, Paessler, Juan Carlos, de la Torre   +2 more
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