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ABSTRACT Mutations in myelin regulatory factor (MYRF) are linked to demyelinating disorders. We report a 38‐year‐old male who developed acute symmetric leukoencephalopathy mimicking a stroke following an influenza A virus infection. While clinical symptoms markedly improved with corticosteroids, MRI revealed persistent white matter lesions, contrasting
Jinghan Hu +5 more
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Evaluation of safety, immunogenicity, and efficacy of inactivated reverse-genetics-based H5N8 highly pathogenic avian influenza virus vaccine with various adjuvants via parenteral and mucosal routes in chickens. [PDF]
Tabynov K +9 more
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Rescuing Newcastle disease virus with tag for screening viral-host interacting proteins based on highly efficient reverse genetics. [PDF]
Wang R +12 more
europepmc +1 more source
Production of OSU G5P[7] Porcine Rotavirus Expressing a Fluorescent Reporter via Reverse Genetics. [PDF]
Snyder AJ, Agbemabiese CA, Patton JT.
europepmc +1 more source
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Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins.
Journal of Visualized Experiments, 2020Rotaviruses are a large and evolving population of segmented double-stranded RNA viruses that cause severe gastroenteritis in the young of many mammalian and avian host species, including humans.
Asha A. Philip +3 more
semanticscholar +1 more source
Forward in Reverse: How Reverse Genetics Complements Chemical Genetics
Pharmacogenomics, 2005Pharmaceutical discovery has a renewed interest in physiological screening, while chemical genomics initiatives will soon provide a large amount of cellular assay data. However, there is no single robust approach to connect active compounds with their targets, limiting their experimental and therapeutic use.
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1999
Abstract The nematode Caenorhabditis elegans has about 19000 genes. Approximately 2000 of these have been identified in forward genetic screens for animals with mutant phenotypes. The generation and analysis of such mutants has been the key to the success of the C. elegans model because the in vivo function of a gene can be inferred from
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Abstract The nematode Caenorhabditis elegans has about 19000 genes. Approximately 2000 of these have been identified in forward genetic screens for animals with mutant phenotypes. The generation and analysis of such mutants has been the key to the success of the C. elegans model because the in vivo function of a gene can be inferred from
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Reverse Genetics of Picornaviruses
1999Publisher Summary This chapter focuses on the reverse genetics of picornaviruses. The family Picornaviridae includes a large number of human and animal pathogens, all related by the possession of a small, nonenveloped, icosahedral particle and a single-stranded, positive-sense, RNA genome.
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Reverse Genetics and Cystic Fibrosis
American Journal of Respiratory Cell and Molecular Biology, 1990The protein responsible for cystic fibrosis has been identified using an approach called "reverse" genetics. This approach relies on the chromosomal map position to direct the search for a disease gene, several novel cloning strategies to isolate the gene, and the gene's sequence to define the abnormal protein.
M C, Iannuzzi, F S, Collins
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