Results 331 to 340 of about 2,173,029 (360)

Reverse Genetics of Mononegavirales

2004
"Reverse genetics" or de novo synthesis of nonsegmented negative-sense RNA viruses (Mononegavirales) from cloned cDNA has become a reliable technique to study this group of medically important viruses. Since the first generation of a negative-sense RNA virus entirely from cDNA in 1994, reverse genetics systems have been established for members of most ...
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Reverse Genetics of Influenza Viruses

2012
The ability to modify influenza viruses at will has revolutionized influenza research. Reverse genetics has been used to generate mutant or reassortant influenza viruses to assess their replication, virulence, pathogenicity, host range, and transmissibility.
Gabriele, Neumann, Makoto, Ozawa, Yoshihiro, Kawaoka   +2 more
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Reverse Genetics Using Transgenic Mice

Annual Review of Physiology, 1990
n, has suggested a genetic approach in which the traditional logic is reversed. In this reverse genetic approach, a genotype is designed and constructed in vitro and implanted in the mouse germline by micro injection or by transfection into embryonic stem cells.
C P, Landel, S Z, Chen, G A, Evans
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Reverse Genetics of Zika Virus

2017
We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A
Chao, Shan, Xuping, Xie, Pei-Yong, Shi
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Prions: Pathogenesis and Reverse Genetics

Annals of the New York Academy of Sciences, 2000
Abstract: Spongiform encephalopathies are a group of infectious neurodegenerative diseases. The infectious agent that causes transmissible spongiform encephalopathies was termed prion by Stanley Prusiner. The prion hypothesis states that the partially protease‐resistant and detergent‐insoluble prion protein (PrPsc) is identical with the infectious ...
Aguzzi, A, Klein, M A, Montrasio, F, Pekarik, V, Brandner, S, Furukawa, H, Käser, P, Röckl, C, Glatzel, M   +8 more
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Rotavirus Reverse Genetics Systems: Development and Application.

Virus Research, 2021
Y. Kanai, Takeshi Kobayashi
semanticscholar   +1 more source

Reverse Genetics Systems for Filoviruses

2017
Filoviruses are among the most pathogenic viruses known to man. Reverse genetics systems, in particular full-length clone systems, allow the generation of recombinant filoviruses, which can be used to study virus biology, but also for applied uses such as screening for countermeasures.
Thomas, Hoenen, Heinz, Feldmann
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Reverse genetics

2023
Olga Baidukova, Simon Kelterborn, Irina Sizova, Peter Hegemann   +3 more
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Genetics in reverse

The Lancet, 2004
Chinnery PF, Cartlidge NEF, Tennant S, Birchall D, Stenhouse SAR   +4 more
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Reverse Genetics for Mammalian Orthoreovirus

2017
Reverse genetics allows introduction of specific alterations into a viral genome. Studies performed with mutant viruses generated using reverse genetics approaches have contributed immeasurably to our understanding of viral replication and pathogenesis, and also have led to development of novel vaccines and virus-based vectors.
Johnasha D, Stuart, Matthew B, Phillips, Karl W, Boehme   +2 more
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