Results 71 to 80 of about 785 (155)

基于ERA-CRISPR/Cas12a-LFD的犀牛特异性检测技术

open access: yes野生动物学报, 2023
野生犀牛(Rhinocerotidae)种群的生存正面临极大威胁,包括栖息地减少、碎片化及偷猎等,在对打击犀牛角等非法贸易的努力中,物种的特异性检测至关重要,为了在现场就可以迅速完成犀牛源性成分鉴定,将酶促重组等温扩增(enzymatic recombinase amplification,ERA)、CRISPR/Cas12a和侧向流试纸条(lateral flow dipstick,LFD)技术相结合,研发了一种适于现场使用的犀牛特异性核酸检测方法。结果表明:该方法只能检测出5种现生犀牛特异性核酸 ...
王瑛   +6 more
doaj  

Seasonal variations in food plant preferences of reintroduced Rhinos Rhinoceros unicornis (Mammalia: Perrissodactyla: Rhinocerotidae) in Manas National Park, Assam, India

open access: yesJournal of Threatened Taxa, 2016
The food preferences of translocated Rhinos in Manas National Park were studied to find out variations in seasonal and annual preferences.  A total of 139 plants species belonging to 39 families were observed to be consumed as food.
Deba Kumar Dutta   +4 more
doaj   +1 more source

Stephanorhinus hundsheimensis (Mammalia, Rhinocerotidae) from the late

open access: yes, 2017
Pleistocene rhinoceroses are poorly documented in Turkey where they have been reported only from the late early Pleistocene (1.3-1.1 Ma) travertine deposits of the Denizli Basin. In this work, new rhinoceros remains collected from this basin are assigned to a relatively large-sized Stephanorhinus hundsheimensis on the basis of their morphology and ...
Pandolfi, L, Erten, H
openaire   +3 more sources

犀牛源性成分PCR检测方法的建立

open access: yes野生动物学报, 2016
为了对犀牛源性成分进行有效鉴定,根据犀科(Rhinocerotidae)动物线粒体DNA(mitochondrial DNA)序列,使用分子生物学软件Oligo 7.0设计了特异性引物,用来建立PCR检测犀牛源性成分的方法。结果显示,建立的PCR方法可以对犀牛阳性样品进行扩增,凝胶电泳呈现特异性条带。方法特异性好,13份其他物种的样品均为阴性结果。方法灵敏度较高,可检测到100拷贝重组质粒DNA模板。对5份送检样品检测发现2份犀牛样品,与测序结果一致。结果表明 ...
邵建宏 赵福振 罗宝正 薄清如 沙才华 廖秀云 徐海聂
doaj  

Capture and transport of white rhinoceroses (Ceratotherium simum) cause shifts in their fecal microbiota composition towards dysbiosis. [PDF]

open access: yesConserv Physiol, 2023
Pohlin F   +10 more
europepmc   +1 more source

Phylogenetically informative proteins from an Early Miocene rhinocerotid. [PDF]

open access: yesNature
Paterson RS   +26 more
europepmc   +1 more source

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