Results 151 to 160 of about 3,061 (189)

Acrylamide-quenching of Rhizomucor miehei lipase

Journal of Photochemistry and Photobiology B: Biology, 2005
Steady-state and time-resolved fluorescence-quenching measurements have been performed to study multitryptophan lipase from filamentous fungus Rhizomucor miehei. Using the steady-state acrylamide fluorescence quenching data and the fluorescence-quenching-resolved-spectra (FQRS) method, the total emission spectrum of native ("closed-lid") lipase has ...
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Activity and stability of a Rhizomucor miehei lipase in hydrophobic media

Biotechnology and Applied Biochemistry, 1997
The effects of detergents and organic solvents on a commercial lipase (Lipozyme) from Rhizomucor miehei were investigated. It was shown that the detergent sodium cholate is possibly an activator of the enzyme, increasing lipase activity 2.5 times (250% of the control) when the enzyme was preincubated with 7 mM cholate.
G M, Dellamora-Ortiz, R C, Martins, W L, Rocha, A P, Dias   +3 more
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Rhizomucor miehei lipase remains highly active at water activity below 0.0001 [PDF]

FEBS Letters, 1992
The lipase from Rhizomucor miehei adsorbed on polymer beads retains substantial catalytic activity even after exhaustive drying, and the use of dry box procedures to prevent entry of atmospheric water.
Peter J. Halling, Macrae, Alasdair R., Rao H. Valivety, Alasdair R. Macrae, Valivety, Rao H., Halling, Peter J.   +5 more
exaly   +2 more sources

An overview of the safety evaluation of theRhizomucor mieheilipase enzyme

Food Additives and Contaminants, 1994
The Rhizomucor miehei lipase enzyme expressed in Aspergillus oryzae, is used in the production of specialty fats, the production of existing fats from new raw materials, or new fats with improved nutritional or functional qualities. It is produced by A. oryzae containing the structural gene for the precursor of R. miehei triglyceride lipase.
A, Broadmeadow, C, Clare, A S, De Boer
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Heat shock response in the thermophilic fungus Rhizomucor miehei

Microbiology, 2014
Changes in the composition of the membrane lipids and cytosol carbohydrates of the thermophilic fungus Rhizomucor miehei in response to heat shock were studied. Under optimal conditions (41–43°C), high trehalose content (8–11%) was found at all stages of growth of submerged culture.
E. A. Yanutsevich, A. S. Memorskaya, N. V. Groza, G. A. Kochkina, V. M. Tereshina   +4 more
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A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Antonie van Leeuwenhoek, 2009
In this study, a beta-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to
Miklós, Takó, Adél, Tóth, László, G Nagy, Judit, Krisch, Csaba, Vágvölgyi, Tamás, Papp   +5 more
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Rhizomucor miehei Aspartic Proteinases Having Improved Properties

1998
Aspartic proteinases are widely spread in most organisms, where they carry out many different functions. The oldest and most well–known use of aspartic proteinases is as coagulants in cheese–making. The coagulants are probably still the only commercial products containing aspartic proteinases as the active substance.
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Molecular modelling studies of substrate binding to the lipase from Rhizomucor miehei

Journal of Computer-Aided Molecular Design, 1997
Lipase enzymes have found increasingly widespread use, especially in biotransformation reactions in organic synthesis. Due to their efficiency and high enantioselectivity, they can be employed in a variety of reactions to carry out asymmetric hydrolyses, esterifications and transesterifications. However, the reasons for their stereospecificity have not
Asutosh T. Yagnik, Jennifer A. Littlechild, Nicholas J. Turner   +2 more
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Biotransformation of oleanolic and maslinic methyl esters by Rhizomucor miehei CECT 2749

Phytochemistry, 2015
The pentacyclic triterpenoids methyl oleanolate, methyl maslinate, methyl 3β-hydroxyolean-9(11),12-dien-28-oate, and methyl 2α,3β-dihydroxy-12β,13β-epoxyolean-28-oate were biotransformed by Rhizomucor miehei CECT 2749. Microbial transformation of methyl oleanolate produced only a 7β,30-dihydroxylated metabolite with a conjugated 9(11),12-diene system ...
Martinez, Antonio, Perojil, Alberto, Rivas, Francisco, Parra, Andres, Garcia-Granados, Andres, Fernandez-Vivas, Antonia   +5 more
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Optimisation of n-octyl oleate enzymatic synthesis over Rhizomucor miehei lipase

Bioprocess and Biosystems Engineering, 2006
Octyl oleate is a useful organic compound with several applications in cosmetic, lubricant and pharmaceutical industry. At first, the enzymatic synthesis of n-octyl oleate by direct lipase-catalysed esterification of oleic acid and 1-octanol was investigated in a stirred batch reactor in solvent-free system.
LAUDANI C. G, HABULIN M, PRIMOZIC M, KNEZ Z, DELLA PORTA, Giovanna, REVERCHON, Ernesto   +5 more
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