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RNA‐Seq for Bacterial Gene Expression [PDF]
Current Protocols in Nucleic Acid Chemistry, 2018 AbstractRNA sequencing (RNA‐seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor‐intensive and expensive part of an RNA‐seq experiment is the preparation of sequencing libraries, which is also essential for the ...
L. D. Poulsen, Jeppe Vinther
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Current Protocols in Molecular Biology, 1993
AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
Michael Z. Gilman, K.J. Reddy
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AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
Michael Z. Gilman, K.J. Reddy
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Autodegradation of RNA of bacterial ribosomes
Canadian Journal of Microbiology, 1973The autodegradation capacity of ribosomes, in the presence or absence of EDTA, has been studied in resting and dividing bacteria. This capacity, in the presence of EDTA, was found to be much lower in the ribosomes of log-phase bacteria when compared to stationary phase bacteria. In absence of EDTA, autodegradation was not observed.
C. Gagnon, G. de Lamirande
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On the biosynthesis of bacterial ribosomal RNA
Molecular Biology Reports, 1973By comparison of the fingerprints of 5S and 23S ribosomal RNAs from Bacillus licheniformis with that of the precursor of 23S ribosomal RNA, it can be shown that 5S RNA is not a part of the precursor of 23S ribosomal RNA.
T. J. Stoof, R. J. Planta
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Cold Spring Harbor Protocols, 2012
In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
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In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
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Current Opinion in Structural Biology, 2001
The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
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The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
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The Decay of Bacterial Messenger RNA
1996Publisher Summary The many demonstrations that the Escherichia coli (E. coli ) rne gene product (RNase E) is involved in messenger RNA (mRNA) decay have given real impetus to the study of this unusual protein's properties and role. The recent attention given to the polyadenylylation of bacterial mRNAs and the discovery that polyadenylylation ...
George J. Murakawa, Donald P. Nierlich
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Bacterial growth physiology and RNA metabolism
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2020Bacteria are sophisticated systems with high capacity and flexibility to adapt to various environmental conditions. Each prokaryote however possesses a defined metabolic network, which sets its overall metabolic capacity, and therefore the maximal growth rate that can be reached. To achieve optimal growth, bacteria adopt various molecular strategies to
Planson, Anne-Gaëlle +3 more
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A Nanostructure Made of a Bacterial Noncoding RNA
Journal of the American Chemical Society, 2009Natural RNAs, unlike many proteins, have never been reported to form extended nanostructures, despite their wide variety of cellular functions. This is all the more striking, as synthetic DNA and RNA forming large nanostructures have long been successfully designed. Here, we show that DsrA, a 87-nt noncoding RNA of Escherichia coli, self-assembles into
Cayrol, Bastien +5 more
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