Rna干涉 - Open Access .click

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Zhongguo shiyan zhenduanxue, 2007
目的探讨RNA干扰技术沉默STAT3基因表达诱导人前列腺癌细胞凋亡的机制。方法针对人STAT3mRNA序列设计合成编码干扰RNA（siRNA）的DNA模板,构建pSilencer 2.1-U6-STAT3-siRNA重组质粒,转染人前列腺癌细胞株PC3,采用Western blot、Northern blot观察STAT3基因表达抑制后Bcl-2,C-Myc与Cyclin D1表达的变化,并用流式细胞技术及吖啶橙染色方法观察细胞凋亡。结果成功构建pSilencer 2.1-U6-STAT3 ...
于浩, 张灵, 高丽芳, 赵雪俭
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丙肝病毒siRNA表达载体构建及抗病毒作用研究

Zhongguo shiyan zhenduanxue, 2009
目的进行丙型肝炎病毒（HCV）RNA干扰（RNA interference RNAi）作用研究。方法本实验选择HCV NS3基因片段作为靶序列,合成含有靶序列的DNA片段并克隆到pGCsi-U6/Neo/GFP/siNeGative载体中,构建可表达具有发夹结构双链siRNA的真核表达质粒pGCsi-U6/Neo/GFP/A,并转染Hela细胞,用HCV攻击该细胞,通过TCID50和HCV负链RT-PCR检测该载体对HCV感染细胞的保护效果。结果通过测序及荧光显微镜观察证明载体构建成功 ...
陈佳玉
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含鼠LINGO-1 shRNA重组腺病毒载体的构建和鉴定

Zhongguo shiyan zhenduanxue, 2009
目的构建含鼠LINGO-1短发夹RNA（short hairpin RNA,shRNA）的复制缺陷型腺病毒载体。方法通过PCR法将带有LINGO-1 shRNA序列构建至U6启动子（U6sh LINGO-1）下游,与T载体连接,将质粒转化入大肠杆菌。将U6sh LINGO-1连到穿梭质粒pDC316上,与腺病毒基因组质粒pBHGlox△E1、3Cre共转染293T细胞,得到重组腺病毒载体（rAd LINGO-1）。经PCR鉴定正确后,进行扩增、纯化、滴度测定及感染性鉴定。结果PCR法得到U6sh ...
高峰, 孙红辉, 杨有庚
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抑制STAT3表达增加H₂O₂诱导人胶质瘤U251细胞损伤

Zhongguo shiyan zhenduanxue, 2010
目的探讨RNA干涉技术抑制STAT3基因表达对过氧化氢（H2O2）复制的人胶质瘤U251细胞损伤模型的影响。方法转染pSilencer1.0-U6-SiRNA-STAT3重组质粒到U251细胞;Western blot方法观察转染重组质粒对STAT3蛋白表达的影响;MTT法检测细胞生存率,TUNEL法检测细胞凋亡,Western blot检测凋亡相关蛋白BCL-2、BAX表达。结果转染pSilencer1.0-U6-SiRNA-STAT3重组质粒能有效抑制U251细胞STAT3蛋白表达（抑制率>50%）;
苏静 +6 more
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[Spatial omics in pulmonary fibrosis: advancing mechanistic insights and therapeutic strategies]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Peng Y, Xie Y, Yang F, Qiao X, Tian Y.
europepmc +1 more source

[Stem cell therapy for premature ovarian insufficiency: a review of clinical evidence and therapeutic strategies]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Cheng Z, Lai D.
europepmc +1 more source

[Role of m6A methylation modification in photoaging and ultraviolet-induced skin cancers]. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban
Hou Y, Zhang X, Liu J, Wu J.
europepmc +1 more source

[Kahweol improves motor function of mice with spinal cord injury by inhibiting microglial activation <i>via</i> regulating the IκBα/NF-κB pathway]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Xia J +6 more
europepmc +1 more source

[Research progress on the roles of primary cilia in fertility]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Yuan Y, Liu R, Huang H.
europepmc +1 more source

[Long noncoding RNA HClnc1 promotes proliferation and migration of liver cancer cells by targeting RBBP5/KAT2B complex to enhance ODC1 transcription]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Feng Z +5 more
europepmc +1 more source