Results 111 to 120 of about 1,323,554 (297)

A combined PIE-FRET and FCS assay to monitor RNA dynamics and cleavage by SARS-CoV-2 Nsp15

Scientific Reports
Nsp15 is an endoribonuclease, highly conserved among coronaviruses, that helps the virus evade detection in host cells by cleaving uridine-rich viral RNA sequences. Those sequences would otherwise trigger immune response pathways.
Kenya Gordon, Zoe M. Wright, Cameron R. Stephens, Meredith N. Frazier, Benjamin S. Clark, Isha M. Wilson, Irene Silvernail, Robin E. Stanley, Sharonda J. LeBlanc   +8 more
doaj   +1 more source

USP7 inhibits FOXO1 activity in chronic lymphocytic leukaemia cells enhancing cell survival

Molecular Oncology, EarlyView.
CLL cells rely on BCR‐mediated signalling for survival and proliferation in part through suppression of FOXO1. FOXO1 activity is regulated by a plethora of post‐translational modifications including phosphorylation and ubiquitination. We demonstrated that dual inhibition of BCR signalling and the deubiquitinase USP7 activates FOXO1, modulating its ...
Hassan N.B. Almuhanna, Jodie Hay, Michael W. Moles, Lucy Clarke, Jennifer Cassels, Alison Sefcick, Alison M. McCaig, Heather G. Jørgensen, Alison M. Michie   +8 more
wiley   +1 more source

TthCsm-mediated DNA cleavage does not require RNA cleavage.

, 2017
(A) TthCsm-mediated cleavage of a complementary ssRNA either with (deoxy-RNA) or without (RNA) deoxynucleotides adjacent to the regularly spaced cleavage sites was tested and analyzed by denaturing PAGE, as in Fig 5C.
Anthony T. Iavarone (331018), Jennifer A. Doudna (253858), Tina Y. Liu (3691906)   +2 more
core   +1 more source

Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer

Molecular Oncology, EarlyView.
New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau, Daniel Pincher, Clifford Young, Nicholas Chappell, Noor A. Lokman, Parul Mittal, Leigh Donnellan, Martin K. Oehler, Doug A. Brooks, Peter Hoffmann   +9 more
wiley   +1 more source

RNA targeting and cleavage by the type III-Dv CRISPR effector complex

Nature Communications
CRISPR-Cas are adaptive immune systems in bacteria and archaea that utilize CRISPR RNA-guided surveillance complexes to target complementary RNA or DNA for destruction1–5.
Evan A. Schwartz, Jack P. K. Bravo, Mohd Ahsan, Luis A. Macias, Caitlyn L. McCafferty, Tyler L. Dangerfield, Jada N. Walker, Jennifer S. Brodbelt, Giulia Palermo, Peter C. Fineran, Robert D. Fagerlund, David W. Taylor   +11 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A conserved NS3 surface patch orchestrates NS2 protease stimulation, NS5A hyperphosphorylation and HCV genome replication.

PLoS Pathogens, 2015
Hepatitis C virus (HCV) infection is a leading cause of liver disease worldwide. The HCV RNA genome is translated into a single polyprotein. Most of the cleavage sites in the non-structural (NS) polyprotein region are processed by the NS3/NS4A serine ...
Olaf Isken, Ulrike Langerwisch, Vlastimil Jirasko, Dirk Rehders, Lars Redecke, Harish Ramanathan, Brett D Lindenbach, Ralf Bartenschlager, Norbert Tautz   +8 more
doaj   +1 more source

Site specific enzymatic cleavage of RNA

Nucleic Acids Research, 1979
The hybridization of a DNA oligonucleotide a specific tetramer or longer) will direct a cleavage by RNase H (EC 3.1.4.34) to a specific site in RNA. The resulting fragments can then be labeled at their 5' or 3' ends, purified, and sequenced directly.
openaire   +3 more sources

Reconstitution of mammalian cleavage factor II involved in 3′ processing of mRNA precursors

, 2018
Cleavage factor II (CF II) is a poorly characterized component of the multiprotein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1.
Treiber, Nora, Meister, Gunter, Wahle, Elmar, Tüting, Christian, Kühn, Uwe, Treiber, Thomas, Graber, Anne, Schönemann, Lars, Sinz, Andrea, Schäfer, Peter, Keller, Walter, Ihling, Christian, Keller W, Kühn U, Treiber N, Tüting C, Wahle E, Graber A, Meister G, Treiber T, Schönemann L, Ihling C, Schäfer P, Sinz A   +23 more
core   +1 more source